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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sherwood, J. B. Shouval, D. |
| Abstract | Establishment of a stable, transformed human renal carcinoma cell line that produces erythropoietin in vitro and has maintained this function continuously since 1981 and for greater than 150 passages in monolayer culture was accomplished by transplantation of human renal clear cell carcinoma tissue from a patient with erythrocytosis into an immunosuppressed athymic mouse. In addition to its immunocrossreactivity with native human urinary erythropoietin, the tumor erythropoietin demonstrates biological activity in the in vitro mouse erythroid colony-forming unit assay and in tumor-bearing nude mice. The cloned renal carcinoma cell line has an abnormal human karyotype and has ultrastructural features characteristic of human renal clear cell carcinoma. This cell line provides a reproducible model system for the production of an erythropoietin-like material and for the study of its synthesis and secretion. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 1 |
| Volume Number | 83 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1986-02-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Erythropoietin Biosynthesis Kidney Neoplasms Metabolism Animals Cell Division Cell Line Clone Cells Colony-Forming Units Assay Erythrocytes Cytology Karyotyping Ultrastructure Mice Mice, Nude Microscopy, Electron Neoplasm Transplantation Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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