NDLI: Identification of a thrombin sequence with growth factor activity on macrophages.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1986 Volume: 83 Issue: 24

Year: 1986 Volume: 83 Issue: 23

Year: 1986 Volume: 83 Issue: 22

Year: 1986 Volume: 83 Issue: 21

Year: 1986 Volume: 83 Issue: 20

Year: 1986 Volume: 83 Issue: 19

Year: 1986 Volume: 83 Issue: 18

Year: 1986 Volume: 83 Issue: 17

Year: 1986 Volume: 83 Issue: 16

Year: 1986 Volume: 83 Issue: 15

Year: 1986 Volume: 83 Issue: 14

Year: 1986 Volume: 83 Issue: 13

Year: 1986 Volume: 83 Issue: 12

Year: 1986 Volume: 83 Issue: 11

Year: 1986 Volume: 83 Issue: 10

Year: 1986 Volume: 83 Issue: 9

Year: 1986 Volume: 83 Issue: 8

Year: 1986 Volume: 83 Issue: 7

Year: 1986 Volume: 83 Issue: 6

Year: 1986 Volume: 83 Issue: 5

Year: 1986 Volume: 83 Issue: 4

A monoclonal antibody that cross-reacts with phosphorylated epitopes on two microtubule-associated proteins and two neurofilament polypeptides.

Cytogenetic 'rogue' cells: what is their frequency, origin, and evolutionary significance?

Colony probing as an alternative to standard sequencing as a means of direct analysis of chromosomal DNA to determine the spectrum of single-base changes in regions of known sequence.

Rabbit and human antibodies to a repeated amino acid sequence of a Plasmodium falciparum antigen, Pf 155, react with the native protein and inhibit merozoite invasion.

Monoclonal antibody MB19 detects genetic polymorphism in human apolipoprotein B.

Small basic proteins of myelin from central and peripheral nervous systems are encoded by the same gene.

Expression of apolipoprotein E during nerve degeneration and regeneration.

Blood platelets express high levels of the pp60c-src-specific tyrosine kinase activity.

Phosphorylation of tyrosine in the carboxyl-terminal tryptic peptide of pp60c-src.

Human small-cell lung cancers show amplification and expression of the N-myc gene.

ATP-binding site of adenylate kinase: mechanistic implications of its homology with ras-encoded p21, F1-ATPase, and other nucleotide-binding proteins.

Molecular cloning and nucleotide sequence of rat kidney gamma-glutamyl transpeptidase cDNA.

Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector.

Similarity between the interleukin 1 receptors on a murine T-lymphoma cell line and on a murine fibroblast cell line.

Presence of abscisic acid, a phytohormone, in the mammalian brain.

Inhibition of splicing but not cleavage at the 5' splice site by truncating human beta-globin pre-mRNA.

Genetic analysis of the fragile-X mental retardation syndrome with two flanking polymorphic DNA markers.

Amplification units containing human N-myc and c-myc genes.

Genetic instability in Drosophila melanogaster: the genetics of an MR element that makes complete P insertion mutations.

Evidence for increased recombination near the human insulin gene: implication for disease association studies.

Elevated erythrocyte adenosine deaminase activity in patients with acquired immunodeficiency syndrome.

Genes involved in Haemophilus influenzae type b capsule expression are part of an 18-kilobase tandem duplication.

Control of the light-regulated current in rod photoreceptors by cyclic GMP, calcium, and l-cis-diltiazem.

Single protein from human leukocytes possesses 5-lipoxygenase and leukotriene A4 synthase activities.

Mapping of phosphomonoester and apparent phosphodiester bonds of the oncogene product p53 from simian virus 40-transformed 3T3 cells.

Structural features of the 5' noncoding region of the rabbit globin messenger RNAs engaged in translation.

Bacteriophage T4 DNA topoisomerase mediates illegitimate recombination in vitro.

Purification and characterization of a membrane-associated 3,3',5-triiodo-L-thyronine binding protein from a human carcinoma cell line.

Are C14-C15 single bond isomerizations of the retinal chromophore involved in the proton-pumping mechanism of bacteriorhodopsin?

Correlation between hormone dependency and the regulation of epidermal growth factor receptor by tumor promoters in human mammary carcinoma cells.

Stimulation of hematopoiesis in vivo by recombinant bacterial murine interleukin 3.

Relationship of VH and VL genes encoding three idiotypic families of anti-p-azobenzenearsonate antibodies.

Decapeptides as effective agonists from L-amino acids biologically equivalent to the luteinizing hormone-releasing hormone.

Facilitating and nonfacilitating synapses on pyramidal cells: a correlation between physiology and morphology.

Bradykinin-activated transmembrane signals are coupled via No or Ni to production of inositol 1,4,5-trisphosphate, a second messenger in NG108-15 neuroblastoma-glioma hybrid cells.

Involvement of the 5'-leader sequence in coupling the stability of a human H3 histone mRNA with DNA replication.

Cocompartmentation of proteins and K+ within the living cell.

Tumor necrosis factor enhances eosinophil toxicity to Schistosoma mansoni larvae.

Secondary activation of c-abl may be related to translocation to the nucleolar organizer region in an in vitro cultured rat leukemia cell line (K3D).

Decreased content of integral membrane calcium-binding protein (IMCAL) in tissues of the spontaneously hypertensive rat.

Operant conditioning of head waving in Aplysia.

Growth and differentiation of cerebellar suspensions transplanted into the adult cerebellum of mice with heredodegenerative ataxia.

Glucocorticoid sensitivity of vasopressin mRNA levels in the paraventricular nucleus of the rat.

Vasoactive intestinal peptide and electrical activity influence neuronal survival.

Theoretical calculation of relative binding affinity in host-guest systems.

Curved DNA: design, synthesis, and circularization.

Role of proline peptide bond isomerization in unfolding and refolding of ribonuclease.

A sialic acid-specific O-acetylesterase in human erythrocytes: possible identity with esterase D, the genetic marker of retinoblastomas and Wilson disease.

RNA splicing products formed with isolated fractions from HeLa cells are associated with fast-sedimenting complexes.

Hypothesis about the function of membrane-buried proline residues in transport proteins.

Lysozyme-induced fusion of liposomes with erythrocyte ghosts at acidic pH.

Identification of a thrombin sequence with growth factor activity on macrophages.

Interrelations between substrate cycles and de novo synthesis of pyrimidine deoxyribonucleoside triphosphates in 3T6 cells.

Focal physiological uncoupling of cerebral blood flow and oxidative metabolism during somatosensory stimulation in human subjects.

Plasma membrane calcium fluxes in intact rods are inconsistent with the 'calcium hypothesis'.

Magnesium sulfate: rationale for its use in preeclampsia.

Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis.

Nerve injury stimulates the secretion of apolipoprotein E by nonneuronal cells.

Evolution in biosynthetic pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region.

Isolation of the gene encoding yeast single-stranded nucleic acid binding protein 1.

alpha-Transducin immunoreactivity in retinae and sensory pineal organs of adult vertebrates.

Anticodon-anticodon interaction induces conformational changes in tRNA: yeast tRNAAsp, a model for tRNA-mRNA recognition.

Mutant ras-encoded proteins with altered nucleotide binding exert dominant biological effects.

Inhibition of tumor-cell-mediated extracellular matrix destruction by a fibroblast proteinase inhibitor, protease nexin I.

Year: 1986 Volume: 83 Issue: 3

Year: 1986 Volume: 83 Issue: 2

Year: 1986 Volume: 83 Issue: 1

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Identification of a thrombin sequence with growth factor activity on macrophages.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wilner, G. D. Bar-shavit, R. Kahn, A. J. Mann, K. G.
Abstract	In contrast to fibroblasts, the exposure of G0/G1-arrested J774 cells, a murine macrophage-like tumor cell line, with either active or esterolytically inactive diisopropyl phosphorofluoridate-conjugated alpha-thrombin (the enzymatically active form of thrombin, EC 3.4.21.5) results in a mitogenic response as measured by increased [3H]thymidine incorporation. This response to thrombin is optimal at 10 nM and is specifically blocked by hirudin, a high-affinity thrombin inhibitor. When prethrombin 1 [a single-chain prothrombin derivative lacking fragment 1, resulting from the action of thrombin on prothrombin] is cleaved with cyanogen bromide, a fragment (peptide CB67-129) is produced that, like the parent thrombin molecule, is mitogenic for J774 cells but not for fibroblasts. Limited tryptic digests of this fragment retain the ability to stimulate macrophages--a function that can be mimicked by a synthetic tetradecapeptide homologue of CB67-129 (representing residues 367-380 of the human thrombin B chain sequence) but not by any of a series of well-known growth promoters, including platelet-derived growth factor, epidermal growth factor, nerve growth factor, and fibroblast epidermal growth factor, nerve growth factor, and fibroblast growth factor. The mitogenic effects of this peptide are not limited to J774 cells but can be expressed in other macrophage-like tumor cell lines, including P388D1, RAW, and PU5. In addition to increased [3H]thymidine incorporation, the synthetic B chain peptide stimulates cell proliferation as evidenced by a dose-dependent increase in total protein per culture well and cell number. We conclude that the thrombin molecule contains a macrophage growth factor domain that is separate and distinct from its active center. Thus, thrombin, in addition to its major role in hemostasis and thrombosis, may also have important functions in such basic processes as the inflammatory response and monocytopoiesis.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	4
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Macrophages Drug Effects Peptide Fragments Pharmacology Thrombin Amino Acid Sequence Animals Cell Division Cell Line Cricetinae Cricetulus DNA Replication Fibroblasts Mice Sequence Homology, Nucleic Acid Stimulation, Chemical Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in