NDLI: Genetic-molecular basis for a simple Drosophila melanogaster somatic system that detects environmental mutagens.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ryo, H. Fujikawa, K. Green, M. M. Todo, T.
Abstract	We have developed a simple, objectively scorable test for the mutagenicity of chemical compounds which can be fed Drosophila melanogaster. The test depends upon the somatic reversion of the X chromosome, recessive eye color mutation, white-ivory (wi) to wild type (w+). Reversions are scored as clones of w+ facets in the wi eyes of eclosing adults. To increase the sensitivity, a tandem quadruplication containing four wi mutations was synthesized. Thus, in homozygous females eight wi mutations are potentially revertible. Six mutagenic compounds, all alkylating agents, all gave positive results at several concentrations tested. Molecular analysis demonstrates that the induced reversions, germinal and somatic, are associated with the loss of 2.9-kilobase DNA duplicated in the wi mutation.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	18
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Environmental Pollutants Toxicity Mutagenicity Tests Animals Drosophila Melanogaster Mutagens Mutation Nucleic Acid Hybridization Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Genetic-molecular basis for a simple Drosophila melanogaster somatic system that detects environmental mutagens.