NDLI: Characterization of mouse mammary tumor virus gag-pro gene products and the ribosomal frameshift site by protein sequencing.

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Characterization of mouse mammary tumor virus gag-pro gene products and the ribosomal frameshift site by protein sequencing.

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Characterization of mouse mammary tumor virus gag-pro gene products and the ribosomal frameshift site by protein sequencing.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sowder, R. C. Henderson, L. E. Oroszlan, S. Hixson, C. V. Hizi, A. Copeland, T. D.
Description	Author Affiliation: Hizi A ( Laboratory of Molecular Virology and Carcinogenesis, Bionetics Research, Inc.-Basic Research Program, National Cancer Institute-Frederick Cancer Research Facility, MD 21701.);
Abstract	The synthesis of retroviral polyproteins that are the translational products of the genome-size mRNA is initiated in the upstream gag gene. The synthesis of the products of the protease gene (pro) and polymerase gene (pol) is regulated by translational suppression (in-frame read-through or frameshift) of termination codons as a strategy developed for controlling the level of replicative enzymes required only in catalytic amounts. In mouse mammary tumor virus (MMTV), three overlapping reading frames are utilized for the synthesis of gag-encoded Pr77, gag-pro-encoded Pr110, and gag-pro-pol-encoded Pr160 polyproteins. To characterize pro gene products and to determine the site of frameshift required for the synthesis of Pr110, we purified and sequenced three MMTV proteins: p14, p30, and p13. Sequence analysis showed that p14 is the basic nucleic acid-binding protein derived entirely from gag, and p13 is a product of the pro gene and has characteristic sequences of protease. A comparison of the amino acid sequences of p30 with the corresponding nucleotide sequence of proviral DNA allowed the delineation of the frameshift site utilized in vivo for the synthesis of the gag-pro-encoded fusion polyprotein Pr110. The results showed that: (i) the N-terminal 94 residues of p30 are translated from the gag frame; (ii) residue leucine-95 is specified by either the last UUG codon of gag or the overlapping CUU codon in the pro frame; and (iii) the elongation of the peptide chain from residue 96 continued to be encoded in the pro frame to the pro terminator. The possible mechanisms of frameshift and of the tRNAs involved are discussed.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	84
Language	English
Publisher	National Academy of Sciences
Publisher Date	1987-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Genes, Overlapping Genes, Viral Genes Mammary Tumor Virus, Mouse Genetics Protein Biosynthesis Retroviridae Proteins Amino Acid Sequence Gene Products, Gag RNA, Messenger RNA, Transfer Physiology RNA, Viral Biosynthesis Isolation & Purification Ribosomes Metabolism Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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