NDLI: Relation of spontaneous transformation in cell culture to adaptive growth and clonal heterogeneity.

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Relation of spontaneous transformation in cell culture to adaptive growth and clonal heterogeneity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yao, A. Rubin, H. Rubin, A. L.
Description	Author Affiliation: Rubin AL ( Department of Molecular and Cell Biology, University of California, Berkeley 94720.);
Abstract	Cell transformation in culture is marked by the appearance of morphologically altered cells that continue to multiply to form discrete foci in confluent sheets when the surrounding cells are inhibited. These foci occur spontaneously in early-passage NIH 3T3 cells grown to confluency in 10% calf serum (CS) but are not seen in cultures grown to confluency in 2% CS. However, repeated passage of the cells at low density in 2% CS gives rise to an adapted population that grows to increasingly higher saturation densities and produces large numbers of foci in 2% CS. The increased saturation density of the adapted population in 2% CS is retained upon repeated passage in 10% CS, but the number and size of the foci produced in 2% CS gradually decrease under this regime. Clonal analysis confirms that the focus-forming potential of most if not all of the cells in a population increases in response to a continuously applied growth constraint, although only a small fraction of the population may actually form foci in a given assay. The acquired capacity for focus formation varies widely in clones derived from the adapted population and changes in diverse ways upon further passage of the clones. We propose that the adaptive changes result from progressive selection of successive phenotypic variations in growth capacity that occur spontaneously. The process designated progressive state selection resolves the apparent dichotomy between spontaneous mutation with selection on the one hand and induction on the other, by introducing selection among fluctuating states or metabolic patterns rather than among genetically altered cells.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	87
Language	English
Publisher	National Academy of Sciences
Publisher Date	1990-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Division Cell Transformation, Neoplastic Cells, Cultured Animals Clone Cells Kinetics Mice Mice, Inbred Strains Time Factors Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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