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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, Y. M. Joseph, D. R. Fenstermacher, D. A. Zahnow, C. A. Sullivan, P. M. Kozak, C. Behrendsen, M. E. |
| Description | Author Affiliation: Joseph DR ( Department of Pediatrics, University of North Carolina, Chapel Hill 27599.); |
| Abstract | Histamine is a neurotransmitter in the central nervous system and an important modulator of gastric acid secretion, vasomotor control, inflammation, and allergic reactions. In biological systems the formation of histamine from its precursor histidine is catalyzed by the enzyme L-histidine decarboxylase (HDC; L-histidine carboxy-lyase, EC 4.1.1.22). We have cloned HDC-encoding cDNA from a fetal rat liver cDNA library (phage lambda gt11) have deduced the amino acid sequence from the nucleotide sequence. The clone was proven to be HDC cDNA by expression of active recombinant enzyme in COS cells and by chromosomal mapping. The cDNA encodes a protein of Mr 73,450 (655 amino acid residues). The discrepancy between this molecular weight and the size of the purified fetal liver protein subunits [Taguchi, Y., Watanabe, T., Kubota, H., Hayashi, H. & Wada, H. (1984) J. Biol. Chem. 259, 5214-5221] (Mr = 54,000) suggests that HDC may be posttranslationally processed. The 469 amino acid residues from the amino-terminal portion of the protein share 50% identity with rat and Drosophila L-dopa decarboxylases and much less homology with other characterized amino acid decarboxylases. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 2 |
| Volume Number | 87 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1990-03-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Carboxy-Lyases Genetics Cloning, Molecular DNA Gene Expression Genes Histidine Decarboxylase Amino Acid Sequence Animals Chromosome Mapping Cricetinae Cricetulus Crosses, Genetic DNA, Recombinant Metabolism Fetus Gene Library Hybrid Cells Enzymology Liver Mice Mice, Inbred Strains Molecular Sequence Data Sequence Homology, Nucleic Acid Comparative Study Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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