NDLI: Conversion of human choriogonadotropin into a follitropin by protein engineering.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1991 Volume: 88 Issue: 24

Year: 1991 Volume: 88 Issue: 23

Year: 1991 Volume: 88 Issue: 22

Year: 1991 Volume: 88 Issue: 21

Year: 1991 Volume: 88 Issue: 20

Year: 1991 Volume: 88 Issue: 19

Year: 1991 Volume: 88 Issue: 18

Year: 1991 Volume: 88 Issue: 17

Year: 1991 Volume: 88 Issue: 16

Year: 1991 Volume: 88 Issue: 15

Year: 1991 Volume: 88 Issue: 14

Year: 1991 Volume: 88 Issue: 13

Year: 1991 Volume: 88 Issue: 12

Year: 1991 Volume: 88 Issue: 11

Year: 1991 Volume: 88 Issue: 10

Year: 1991 Volume: 88 Issue: 9

Year: 1991 Volume: 88 Issue: 8

Year: 1991 Volume: 88 Issue: 7

Year: 1991 Volume: 88 Issue: 6

Year: 1991 Volume: 88 Issue: 5

Year: 1991 Volume: 88 Issue: 4

Year: 1991 Volume: 88 Issue: 3

Measuring time to the doctorate: reinterpretation of the evidence.

Transition from one revolving cluster to two revolving clusters in the ground-state rotational bands of nuclei in the lanthanon region.

Root communication among desert shrubs.

Is the cygnet the quintessential baryon?

CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the A alpha chain of fibrinogen.

Glutamate and 2-amino-4-phosphonobutyrate evoke an increase in potassium conductance in retinal bipolar cells.

Continuous microspectrophotometric measurement of DNA polymerase activity: application to the Klenow fragment of Escherichia coli DNA polymerase I and human immunodeficiency virus type 1 reverse transcriptase.

Cloning and expression of a rat brain alpha 2B-adrenergic receptor.

FKB1 encodes a nonessential FK 506-binding protein in Saccharomyces cerevisiae and contains regions suggesting homology to the cyclophilins.

Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2.

Precerebellin is a cerebellum-specific protein with similarity to the globular domain of complement C1q B chain.

Effects of binocular deprivation on the development of clustered horizontal connections in cat striate cortex.

GTPase-activating protein interactions with the viral and cellular Src kinases.

Cardiac fibroblasts are predisposed to convert into myocyte phenotype: specific effect of transforming growth factor beta.

Rapid induction of the c-jun protooncogene in the avian oviduct by the antiestrogen tamoxifen.

Cloning of murine ferrochelatase.

Molecular cloning and chromosomal localization of the murine homolog of the human helix-loop-helix gene SCL.

A 45-kDa protein antigenically related to band 3 is selectively expressed in kidney mitochondria.

Suppression of human immunodeficiency virus expression in chronically infected monocytic cells by glutathione, glutathione ester, and N-acetylcysteine.

Direct and synergistic effects of interleukin 11 on murine hemopoiesis in culture.

Polarized expression of cytokines in cell conjugates of helper T cells and splenic B cells.

Spontaneous solubilization of membrane-bound human testis angiotensin-converting enzyme expressed in Chinese hamster ovary cells.

Capture of flanking DNA by a P element in Drosophila melanogaster: creation of a transposable element.

Binary system for regulating transgene expression in mice: targeting int-2 gene expression with yeast GAL4/UAS control elements.

Calcium channels from Cyprinus carpio skeletal muscle.

Herpes simplex virus latency-associated transcript is a stable intron.

cAMP stimulation of acetylcholine receptor expression is mediated through posttranslational mechanisms.

Molecular basis of the activation of the tumorigenic potential of Gag-insulin receptor chimeras.

Heme-protein fission under nondenaturing conditions.

Cyclic AMP mediates the direct antiproliferative action of mismatched double-stranded RNA.

cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera.

Single-step selection for Ty1 element retrotransposition.

The transcriptional transactivator of human foamy virus maps to the bel 1 genomic region.

Free radical-derived quinone methide mediates skin tumor promotion by butylated hydroxytoluene hydroperoxide: expanded role for electrophiles in multistage carcinogenesis.

Transgenic mice susceptible to poliovirus.

Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains.

Insect immune response to bacterial infection is mediated by eicosanoids.

In vivo T-cell activation by staphylococcal enterotoxin B prevents outgrowth of a malignant tumor.

Depletion of extrinsic pathway inhibitor (EPI) sensitizes rabbits to disseminated intravascular coagulation induced with tissue factor: evidence supporting a physiologic role for EPI as a natural anticoagulant.

Conversion of human choriogonadotropin into a follitropin by protein engineering.

Genetic and molecular characterization of tube, a Drosophila gene maternally required for embryonic dorsoventral polarity.

Purification of the active C5a receptor from human polymorphonuclear leukocytes as a receptor-Gi complex.

Specific initiation by RNA polymerase I in a whole-cell extract from yeast.

Accurate and efficient RNA polymerase II transcription with a soluble nuclear fraction derived from Drosophila embryos.

Temporal and spatial patterns of transgene expression in aging adult mice provide insights about the origins, organization, and differentiation of the intestinal epithelium.

Heterochromatic features of an 11-megabase transgene in brain cells.

Recombinant fusion protein identified by lepromatous sera mimics native Mycobacterium leprae in T-cell responses across the leprosy spectrum.

The role of envelope proteins in hepatitis B virus assembly.

Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu.

Structural analysis of the interaction between the human immunodeficiency virus Rev protein and the Rev response element.

Regulation of ferritin and heme oxygenase synthesis in rat fibroblasts by different forms of iron.

Phosphoramidon blocks the pressor activity of porcine big endothelin-1-(1-39) in vivo and conversion of big endothelin-1-(1-39) to endothelin-1-(1-21) in vitro.

Negative supercoiling of DNA facilitates an interaction between transcription factor IID and the fibroin gene promoter.

Photoreceptor peripherin is the normal product of the gene responsible for retinal degeneration in the rds mouse.

Sequence requirement for peptide recognition by rat brain p21ras protein farnesyltransferase.

Cord factor (alpha,alpha-trehalose 6,6'-dimycolate) inhibits fusion between phospholipid vesicles.

Electron probe microanalysis of calcium release and magnesium uptake by endoplasmic reticulum in bee photoreceptors.

Signal transduction in bacteria: CheW forms a reversible complex with the protein kinase CheA.

Mutated recombinant human heavy-chain ferritins and myelosuppression in vitro and in vivo: a link between ferritin ferroxidase activity and biological function.

Cholinergic depletion prevents expansion of topographic maps in somatosensory cortex.

Expression of a conserved cell-type-specific protein in nerve terminals coincides with synaptogenesis.

Identification of a T-cell-specific transcriptional enhancer located 3' of C gamma 1 in the murine T-cell receptor gamma locus.

Splice-mediated insertion of an Alu sequence inactivates ornithine delta-aminotransferase: a role for Alu elements in human mutation.

Comparison of long and short forms of the prolactin receptor on prolactin-induced milk protein gene transcription.

Propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting.

Drift, admixture, and selection in human evolution: a study with DNA polymorphisms.

Inhibition of growth of experimental prostate cancer with sustained delivery systems (microcapsules and microgranules) of the luteinizing hormone-releasing hormone antagonist SB-75.

Expression of the bacterioopsin gene in Halobacterium halobium using a multicopy plasmid.

Homologous recombination in Leishmania enriettii.

Molecular localization of the t(11;22)(q24;q12) translocation of Ewing sarcoma by chromosomal in situ suppression hybridization.

Model for the structure of the lipid bilayer.

In vitro neurons in mammalian cortical layer 4 exhibit intrinsic oscillatory activity in the 10- to 50-Hz frequency range.

Thyrotropin-luteinizing hormone/chorionic gonadotropin receptor extracellular domain chimeras as probes for thyrotropin receptor function.

Mutational activation of the c-Ha-ras gene in liver tumors of different rodent strains: correlation with susceptibility to hepatocarcinogenesis.

Kinetics of self-assembly of alpha alpha-tropomyosin coiled coils from unfolded chains.

CGG: an unassigned or nonsense codon in Mycoplasma capricolum.

Guanosine 5'-[beta-thio]triphosphate selectively activates calcium signaling in mast cells.

Molecular genetic basis for complex flagellar antigen expression in a triphasic serovar of Salmonella.

Promotion of central cholinergic and dopaminergic neuron differentiation by brain-derived neurotrophic factor but not neurotrophin 3.

Cloning of the DNA-binding subunit of human nuclear factor kappa B: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha.

Sequence determination and modeling of structural motifs for the smallest monomeric aminoacyl-tRNA synthetase.

Peptide-induced antiviral protection by cytotoxic T cells.

Year: 1991 Volume: 88 Issue: 2

Year: 1991 Volume: 88 Issue: 1

Two-dimensional life?

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Conversion of human choriogonadotropin into a follitropin by protein engineering.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Campbell, R. K. Dean-emig, D. M. Moyle, W. R.
Description	Author Affiliation: Campbell RK ( Department of Obstetrics & Gynecology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854.);
Abstract	Human reproduction is dependent upon the actions of follicle-stimulating hormone (hFSH), luteinizing hormone (hLH), and chorionic gonadotropin (hCG). While the alpha subunits of these heterodimeric proteins can be interchanged without effect on receptor-binding specificity, their beta subunits differ and direct hormone binding to either LH/CG or FSH receptors. Previous studies employing chemical modifications of the hormones, monoclonal antibodies, or synthetic peptides have implicated hCG beta-subunit residues between Cys-38 and Cys-57 and corresponding regions of hLH beta and hFSH beta in receptor recognition and activation. Since the beta subunits of hCG or hLH and hFSH exhibit very little sequence similarity in this region, we postulated that these residues might contribute to hormone specificity. To test this hypothesis we constructed chimeric hCG/hFSH beta subunits, coexpressed them with the human alpha subunit, and examined their ability to interact with LH and FSH receptors and hormone-specific monoclonal antibodies. Surprisingly, substitution of hFSH beta residues 33-52 for hCG beta residues 39-58 had no effect on receptor binding or stimulation. However, substitution of hFSH beta residues 88-108 in place of the carboxyl terminus of hCG beta (residues 94-145) resulted in a hormone analog identical to hFSH in its ability to bind and stimulate FSH receptors. The altered binding specificity displayed by this analog is not attributable solely to the replacement of hCG beta residues 108-145 or substitution of residues in the 'determinant loop' located between hCG beta residues 93 and 100.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	88
Language	English
Publisher	National Academy of Sciences
Publisher Date	1991-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Chorionic Gonadotropin Genetics Follicle Stimulating Hormone Genetic Engineering Mutagenesis, Insertional Amino Acid Sequence Animals Cell Line Metabolism Pharmacology Corpus Luteum Kinetics Leydig Cells Drug Effects Molecular Sequence Data Plasmids Receptors, LH Recombinant Proteins Sequence Homology, Nucleic Acid Testosterone Biosynthesis Transfection Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in