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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Link, P. Aponte, G. W. Halldén, G. Hess, R. Keddie, A. |
| Description | Author Affiliation: Aponte GW ( Department of Nutritional Sciences, University of California, Berkeley 94720.); |
| Abstract | A cell culture system has been developed that produces stable gastrointestinal (GI) polarized cell lines capable of maintaining hormone secretion. A spontaneously transformed rat mucosal epithelial cell was selected for hypoxanthine/guanine phosphoribosyltransferase deficiency and transfected with a plasmid conferring hygromycin resistance (BRIE 291 cells). Fusion of these cells with dispersed small intestinal epithelia cells resulted in hybrid cell lines that retained characteristic properties of the native GI cell more effectively than the transformed tumorigenic parental cell line. Hybrid hBRIE 380 cells are uniformly cuboidal with microvilli, contain villin, are contact inhibited, are anchorage dependent, require serum supplementation for growth, and are more sensitive to virus infection than the parental BRIE 291 cells. Fusion of BRIE 291 with dispersed pancreatic islet cells has resulted in a variety of pancreatic-hormone-producing cell lines. One of these, hybrid hBRIE 291-i2, forms confluent monolayers capable of synthesizing insulin-like immunoreactivity. These studies demonstrate that functionally polarized GI cells can be generated from primary cultures of nondividing committed epithelial cells by somatic cell hybridization and make feasible the selection and maintenance of specific GI epithelial cell types in confluent monolayer cultures. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 12 |
| Volume Number | 88 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1991-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Intestinal Mucosa Cytology Animals Carrier Proteins Metabolism Cell Line, Transformed Electrophoresis, Polyacrylamide Gel Hormones Hybrid Cells Hypoxanthine Phosphoribosyltransferase Deficiency Immunohistochemistry Insulin Enzymology Ultrastructure Islets Of Langerhans Keratins Microfilament Proteins Rats, Inbred Strains Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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