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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Skriver, K. Rogers, J. C. Mundy, J. Olsen, F. L. |
| Description | Author Affiliation: Skriver K ( Carlsberg Research Laboratory Copenhagen, Denmark.); |
| Abstract | We have used a transient expression assay in aleurone protoplasts of barley to delineate hormone response elements of the abscisic acid (ABA)-responsive rice gene Rab16A and of the gibberellin A3 (GA3)-responsive barley alpha-amylase gene Amy 1/6-4. Our approach used transcriptional fusions between their 5' upstream sequences and a bacterial chloramphenicol acetyltransferase reporter gene. A chimeric promoter containing six copies of the -181 to -171 region of Rab 16A fused to a minimal promoter conferred ABA-responsive expression on the reporter gene. Transcription from this ABA response element (GTACGTGGCGC) was unaffected by GA3. A chimeric promoter containing six copies of the -148 to -128 sequence of Amy 1/6-4 fused to the minimal promoter conferred GA3-responsive expression on the reporter gene. Transcription from this GA3 response element (GGCCGATAACAAACTCCGGCC) was repressed by ABA. The effect on transcription from both hormone response elements was orientation-independent, indicating that they function as inducible enhancers in their native genes. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 16 |
| Volume Number | 88 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1991-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Abscisic Acid Pharmacology DNA Genetics Genes, Plant Drug Effects Gibberellins Hordeum Oryza Sativa Transcription, Genetic Chloramphenicol O-Acetyltransferase Metabolism Enzymology Molecular Sequence Data Promoter Regions, Genetic Protoplasts Physiology Recombinant Proteins TATA Box Transfection Alpha-Amylases Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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