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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Gubler, F. Jacobsen, J. V. |
| Description | Country affiliation: Australia Author Affiliation: Gubler F ( Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, Canberra, ACT, Australia.) |
| Abstract | Deletion analysis has previously shown that the major gibberellic acid (GA)- and abscisic acid (ABA)-responsive elements in the promoter of a high-pI alpha-amylase gene of barley are located downstream of -174 (Jacobsen and Close, 1991). We have used transient expression assays in barley aleurone protoplasts to identify sequences between -174 and +53 that confer GA and ABA responsiveness on expression of a beta-glucuronidase reporter gene. Using alpha-amylase promoter fragments and synthetic oligonucleotides fused to minimal promoters, we have shown that the hormone-responsive region is located between -174 and -108. A single copy of this region fused to a minimal alpha-amylase promoter (-41) conferred both GA- and ABA-responsive expression on the reporter gene comparable to the positive control, Am(-174)IGN. Multiple copies of this region were able to activate even greater levels of expression. Site-directed mutagenesis was used to determine the functional importance of the conserved motifs (-169pyrimidine box, -143TAACAAA box, and -124TATCCAC box) and nonconserved intervening sequences within the region between -174 and -108. Our results showed that both the TAACAAA and TATCCAC boxes play an important role in GA-regulated expression. We propose that the TAACAAA box is a gibberellin response element, that the TATCCAC box acts cooperatively with the TAACAAA box to give a high level of GA-regulated expression, and that together these motifs form important components of a gibberellin response complex in high-pI alpha-amylase genes. The TAACAAA box also appears to be the site of action of ABA.(ABSTRACT TRUNCATED AT 250 WORDS) |
| File Format | HTM / HTML |
| ISSN | 10404651 |
| e-ISSN | 1531298X |
| Journal | THE PLANT CELL ONLINE |
| Issue Number | 11 |
| Volume Number | 4 |
| Language | English |
| Publisher | American Society of Plant Biologists |
| Publisher Date | 1992-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Botany Genes, Plant Genetics Gibberellins Pharmacology Hordeum Promoter Regions, Genetic Alpha-amylases Dna Mutational Analysis Dna, Recombinant Drug Effects Enzymology Molecular Sequence Data Mutagenesis, Site-directed Structure-activity Relationship Transcription, Genetic |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Plant Science |
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