NDLI: Specific inhibition of transcription by triple helix-forming oligonucleotides.

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Specific inhibition of transcription by triple helix-forming oligonucleotides.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hélène, C. Thuong, N. T. Duval-valentin, G.
Description	Author Affiliation: Duval-Valentin G ( Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, Institut National de la Santé et de la Recherche Médicale Unité 201, Centre National de la Recherche Scientifique, Paris, France.);
Abstract	Homopyrimidine oligonucleotides bind to the major groove of a complementary homopyrimidine.homopurine stretch by triple helix formation. The bla gene from transposon Tn3 contains a homopyrimidine.homopurine sequence of 13 base pairs located just downstream of the RNA polymerase binding site. A 13-mer homopyrimidine oligonucleotide targeted to this sequence was tested for its effect on transcription of the bla gene in vitro. We show that the consequence of triple helix formation in front of the Escherichia coli RNA polymerase-promoter complex is to block the holoenzyme at its start site during a period that is dependent on temperature. The temperature dependence of transcription inhibition shows a direct correlation between this effect and the stabilization of the triple helix. Substitution of 5-methylcytosine to cytosine in the 13-mer oligonucleotide enhances triplex stability and transcription inhibition. Transcription inhibition by this synthetic repressor was also confirmed by footprinting studies demonstrating its specificity of action. The 13-mer oligonucleotide containing a psoralen derivative covalently linked to its 5' end shows an irreversible and specific inhibition of transcription initiation after exposure to light of wavelength greater than 310 nm.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	2
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA-Directed RNA Polymerases Metabolism Oligodeoxyribonucleotides Pharmacology Transcription, Genetic Drug Effects Deoxyribonuclease I Escherichia Coli Genetics Furocoumarins Chemistry In Vitro Techniques Molecular Sequence Data Molecular Structure Nucleic Acid Conformation Promoter Regions, Genetic Structure-Activity Relationship Temperature Time Factors Beta-Lactamases Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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