NDLI: Minimal sequence requirements for ribozyme activity.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1992 Volume: 89 Issue: 24

Year: 1992 Volume: 89 Issue: 23

Year: 1992 Volume: 89 Issue: 22

Year: 1992 Volume: 89 Issue: 21

Year: 1992 Volume: 89 Issue: 20

Year: 1992 Volume: 89 Issue: 19

Year: 1992 Volume: 89 Issue: 18

Year: 1992 Volume: 89 Issue: 17

Year: 1992 Volume: 89 Issue: 16

Year: 1992 Volume: 89 Issue: 15

Year: 1992 Volume: 89 Issue: 14

Year: 1992 Volume: 89 Issue: 13

Inhibition of assimilatory nitrate reductase activity in soil by glutamine and ammonium analogs.

Intracellular amplification and expression of a synthetic analog of rotavirus genomic RNA bearing a foreign marker gene: mapping cis-acting nucleotides in the 3'-noncoding region.

Cloned human neuropeptide Y receptor couples to two different second messenger systems.

Viral E1 and E2 proteins support replication of homologous and heterologous papillomaviral origins.

Functional binding of the 'TATA' box binding component of transcription factor TFIID to the -30 region of TATA-less promoters.

Human chromosomal localization of genes encoding the gamma 1 and gamma 2 subunits of the gamma-aminobutyric acid receptor indicates that members of this gene family are often clustered in the genome.

Recognition of the Epstein-Barr virus-encoded nuclear antigens EBNA-4 and EBNA-6 by HLA-A11-restricted cytotoxic T lymphocytes: implications for down-regulation of HLA-A11 in Burkitt lymphoma.

Membrane-bound Dictyostelium myosin heavy chain kinase: a developmentally regulated substrate-specific member of the protein kinase C family.

Hormonal regulation of the thyrotropin beta-subunit gene by phosphorylation of the pituitary-specific transcription factor Pit-1.

Transient immunoglobulin M antibody response to hepatitis C virus capsid antigen in posttransfusion hepatitis C: putative serological marker for acute viral infection.

Oxytocin receptor distribution reflects social organization in monogamous and polygamous voles.

A dominant mutation in the Wilms tumor gene WT1 cooperates with the viral oncogene E1A in transformation of primary kidney cells.

Rapid fitness losses in mammalian RNA virus clones due to Muller's ratchet.

Molecular cloning of a Drosophila diacylglycerol kinase gene that is expressed in the nervous system and muscle.

High rates of Ac/Ds germinal transposition in Arabidopsis suitable for gene isolation by insertional mutagenesis.

Vitamin D-responsive protein-DNA interactions at multiple promoter regulatory elements that contribute to the level of rat osteocalcin gene expression.

Parental bias of Ki-ras oncogenes detected in lung tumors from mouse hybrids.

Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody.

Recombinant anti-erbB2 immunotoxins containing Pseudomonas exotoxin.

Design and synthesis of a CD4 beta-turn mimetic that inhibits human immunodeficiency virus envelope glycoprotein gp120 binding and infection of human lymphocytes.

Nitric oxide stimulates the ADP-ribosylation of a 41-kDa cytosolic protein in Dictyostelium discoideum.

Isolation of a gene encoding a chaperonin-like protein by complementation of yeast amino acid transport mutants with human cDNA.

High-efficiency receptor-mediated delivery of small and large (48 kilobase gene constructs using the endosome-disruption activity of defective or chemically inactivated adenovirus particles.

Oncogenic point mutations in exon 20 of the RB1 gene in families showing incomplete penetrance and mild expression of the retinoblastoma phenotype.

Genetic, cytogenetic, and molecular analyses of mutations induced by melphalan demonstrate high frequencies of heritable deletions and other rearrangements from exposure of postspermatogonial stages of the mouse.

Laser desorption studies of high mass biomolecules in Fourier-transform ion cyclotron resonance mass spectrometry.

Tyrosine phosphorylation of G protein alpha subunits by pp60c-src.

In vivo production of a stable single-stranded cDNA in Saccharomyces cerevisiae by means of a bacterial retron.

Identification of Tyr-185 as the site of tyrosine autophosphorylation of recombinant mitogen-activated protein kinase p42mapk.

Epitope-specific suppression of antibody response in experimental autoimmune myasthenia gravis by a monomethoxypolyethylene glycol conjugate of a myasthenogenic synthetic peptide.

Detection of rare antigen-presenting cells by the lacZ T-cell activation assay suggests an expression cloning strategy for T-cell antigens.

Synthesis of mannosylglucosaminylinositol phospholipids in normal but not paroxysmal nocturnal hemoglobinuria cells.

Adhesion is required for protein kinase C-dependent activation of the Na+/H+ antiporter by platelet-derived growth factor.

Cloning, expression, and crystallization of recoverin, a calcium sensor in vision.

Changing patterns in cytoskeletal mRNA expression and protein synthesis during murine erythropoiesis in vivo.

Primary structures of chicken cone visual pigments: vertebrate rhodopsins have evolved out of cone visual pigments.

Antithrombotic effects of synthetic peptides targeting various functional domains of thrombin.

Differential regulation of T helper phenotype development by interleukins 4 and 10 in an alpha beta T-cell-receptor transgenic system.

Phosphatidylinositol-glycan (PI-G)-anchored membrane proteins: requirement of ATP and GTP for translation-independent COOH-terminal processing.

Contraction characteristics and ATPase activity of skeletal muscle fibers in the presence of antibody to myosin subfragment 2.

Protein F, a fibronectin-binding protein, is an adhesin of the group A streptococcus Streptococcus pyogenes.

Atomic structures of wild-type and thermostable mutant recombinant human Cu,Zn superoxide dismutase.

Surface-bound optical probes monitor protein translocation and surface potential changes during the bacteriorhodopsin photocycle.

Minimal sequence requirements for ribozyme activity.

In vitro mutagenesis of biologically active transcripts of beet necrotic yellow vein virus RNA 2: evidence that a domain of the 75-kDa readthrough protein is important for efficient virus assembly.

Stable transformation of a mosquito cell line results in extraordinarily high copy numbers of the plasmid.

Organ injury associated with extrathymic induction of immune tolerance in doubly transgenic mice.

A simple explanation of the electrostatics of the B-to-Z transition of DNA.

[Val12] HRAS downregulates GLUT2 in beta cells of transgenic mice without affecting glucose homeostasis.

High-speed platelet adhesion under conditions of rapid flow.

Evaluation of luteinizing hormone-releasing hormone antagonistic activity in vitro.

Regulation of immediate early gene expression and AP-1 binding in the rat nucleus accumbens by chronic cocaine.

Gene expression in cotton (Gossypium hirsutum L.) fiber: cloning of the mRNAs.

On the definition of the concepts thinking, consciousness, and conscience.

Protein interaction cloning in yeast: identification of mammalian proteins that react with the leucine zipper of Jun.

In vivo protein-DNA interactions at hypersensitive site 3 of the human beta-globin locus control region.

Expression of the cholecystokinin gene in pediatric tumors.

Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA1 are resistant to virus infection.

Identification of the major soluble cuticular glycoprotein of lymphatic filarial nematode parasites (gp29) as a secretory homolog of glutathione peroxidase.

Heterotrimeric G proteins in synaptoneurosome membranes are crosslinked by p-phenylenedimaleimide, yielding structures comparable in size to crosslinked tubulin and F-actin.

Whole genome amplification from a single cell: implications for genetic analysis.

Determinant nucleotides of yeast tRNA(Asp) interact directly with aspartyl-tRNA synthetase.

Functional conversion of the homologous proteins alpha-lactalbumin and lysozyme by exon exchange.

Fumarase C, the stable fumarase of Escherichia coli, is controlled by the soxRS regulon.

Specific binding of the diphtheria tox regulatory element DtxR to the tox operator requires divalent heavy metal ions and a 9-base-pair interrupted palindromic sequence.

Clonal proliferation of murine lymphohemopoietic progenitors in culture.

Nonconservative recombination in Escherichia coli.

Two mutations in Marfan syndrome resulting in truncated fibrillin polypeptides.

Multiple mechanisms mediate glucose repression of the yeast GAL1 gene.

Developmental failure of chimeric embryos expressing high levels of H-2Dd transplantation antigens.

Side-chain entropy opposes alpha-helix formation but rationalizes experimentally determined helix-forming propensities.

Characterization of human androgen receptor overexpressed in the baculovirus system.

Intrinsic signal changes accompanying sensory stimulation: functional brain mapping with magnetic resonance imaging.

The cytoplasmic component of the bacterial flagellar motor.

Estimating substitution rates from molecular data using the coalescent.

A promoter element that exerts positive and negative control of the interleukin 2-responsive J-chain gene.

Identification and molecular characterization of a Salmonella typhimurium gene involved in triggering the internalization of salmonellae into cultured epithelial cells.

Cloning of a cuticular antigen that contains multiple tandem repeats from the filarial parasite Dirofilaria immitis.

Binding of the Bacillus subtilis spoIVCA product to the recombination sites of the element interrupting the sigma K-encoding gene.

Early persistent activation of sperm K+ channels by the egg peptide speract.

Folate receptor allows cells to grow in low concentrations of 5-methyltetrahydrofolate.

The diabetogenic effects of glucocorticoids are more pronounced in low- than in high-insulin responders.

The deep, hot biosphere.

Biosynthesis and endocrine control of the production of the German cockroach sex pheromone 3,11-dimethylnonacosan-2-one.

Localized torsional tension in the DNA of human cells.

Beta 2-microglobulin-, CD8+ T-cell-deficient mice survive inoculation with high doses of vaccinia virus and exhibit altered IgG responses.

Beta-amyloid precursor protein cleavage by a membrane-bound protease.

Crystal structure of rat liver dihydropteridine reductase.

Parametric sequence comparisons.

Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes.

Cloning of a mu-class glutathione S-transferase gene and identification of the glucocorticoid regulatory domains in its 5' flanking sequence.

Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase.

Reconstruction of a Streptomyces linear replicon from separately cloned DNA fragments: existence of a cryptic origin of circular replication within the linear plasmid.

The endoplasmic reticulum-sarcoplasmic reticulum connection: distribution of endoplasmic reticulum markers in the sarcoplasmic reticulum of skeletal muscle fibers.

Cytosolic phospholipase A2 is coupled to hormonally regulated release of arachidonic acid.

Enhancer of splitD, a dominant mutation of Drosophila, and its use in the study of functional domains of a helix-loop-helix protein.

Transfer of multiple sclerosis into severe combined immunodeficiency mice by mononuclear cells from cerebrospinal fluid of the patients.

Changes in intracellular calcium during the development of epithelial polarity and junctions.

DHR3: a Drosophila steroid receptor homolog.

The complete sequence of Drosophila beta-spectrin reveals supra-motifs comprising eight 106-residue segments.

Rapid decline of chronic myeloid leukemic cells in long-term culture due to a defect at the leukemic stem cell level.

Selective stimulation of prostatic carcinoma cell proliferation by transferrin.

pH-dependent insertion of proteins into membranes: B-chain mutation of diphtheria toxin that inhibits membrane translocation, Glu-349----Lys.

Reversion of recombinant toxoids: mutations in diphtheria toxin that partially compensate for active-site deletions.

Characterization of a periplasmic thiol:disulfide interchange protein required for the functional maturation of secreted virulence factors of Vibrio cholerae.

Fragmentation of proteins in the 13- to 29-kDa mass range observed by 252Cf-plasma desorption mass spectrometry.

Interaction between G-protein beta and gamma subunit types is selective.

Year: 1992 Volume: 89 Issue: 12

Year: 1992 Volume: 89 Issue: 11

Year: 1992 Volume: 89 Issue: 10

Year: 1992 Volume: 89 Issue: 9

Year: 1992 Volume: 89 Issue: 8

Year: 1992 Volume: 89 Issue: 7

Year: 1992 Volume: 89 Issue: 6

Year: 1992 Volume: 89 Issue: 5

Year: 1992 Volume: 89 Issue: 4

Year: 1992 Volume: 89 Issue: 3

Year: 1992 Volume: 89 Issue: 2

Year: 1992 Volume: 89 Issue: 1

On the nature of global classification.

Theory of hydrophobicity: transient cavities in molecular liquids.

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Minimal sequence requirements for ribozyme activity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mccall, M. J. Hendry, P. Jennings, P. A.
Description	Author Affiliation: McCall MJ ( Commonwealth Scientific and Industrial Research Organization, Division of Biomolecular Engineering, North Ryde, NSW, Australia.);
Abstract	The hammerhead ribozyme, as engineered by J. Haseloff and W. L. Gerlach [(1988) Nature (London) 334, 585-591], is an RNA molecule containing two regions of conserved nucleotides, a double helix, called helix II, which connects the two conserved regions, and flanking arms of variable sequence, which hybridize the ribozyme to its specific target. Here we show that this ribozyme may be reduced in size and still retain cleavage activity by replacing helix II with just a few nucleotides that cannot form Watson-Crick base pairs between themselves. Furthermore, the nucleotides replacing helix II and the nucleotides in the flanking arms may be substituted with DNA, and this small, DNA-containing ribozyme is fully as active as the original, full-size ribozyme. Cleavage activity of the minimized ribozyme depends on the number and sequence of the few nucleotides that replace helix II; optimal activity, thus far, is achieved by four or five deoxyribopyrimidines. The minimized ribozyme, or 'minizyme,' is active as a monomer, as shown by its nearly constant activity over a concentration range varying 25,000-fold, by the mobility of the minizyme-substrate complex in nondenaturing polyacrylamide gels as compared with other nucleic acid molecules of known size, and by other observations. These minizymes provide an excellent model system for studying the structure and mechanism of catalytic RNA; they might also be useful in a variety of biological applications.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	13
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	RNA, Catalytic Chemistry Kinetics Molecular Sequence Data Molecular Structure Oligodeoxyribonucleotides Oligonucleotides Structure-Activity Relationship Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in