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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pellegrini, G. Lorenzet, R. Furie, B. C. Ready, N. Furie, B. Celi, A. De Blasi, A. |
| Description | Author Affiliation: Celi A ( Center for Hemostasis and Thrombosis Research, New England Medical Center, Boston, MA 02111.); |
| Abstract | P-selectin on activated platelets and stimulated endothelial cells mediates cell adhesion with monocytes and neutrophils. Since activated platelets induce tissue factor on mononuclear leukocytes, we examined the effect of P-selectin on the expression of tissue factor activity in monocytes. Purified P-selectin stimulated tissue factor expression on mononuclear leukocytes in a dose-dependent manner. Chinese hamster ovary (CHO) cells expressing P-selectin stimulated tissue factor procoagulant activity in purified monocytes, whereas untransfected CHO cells and CHO cells expressing E-selectin did not. Anti-P-selectin antibodies inhibited the effects of purified P-selectin and CHO cells expressing P-selectin on monocytes. Incubation of CHO cells expressing P-selectin with monocytes leads to the development of tissue factor mRNA in monocytes and to the expression of tissue factor antigen on the monocyte surface. These results indicate that P-selectin upregulates the expression of tissue factor on monocytes as well as mediates the binding of platelets and endothelial cells with monocytes and neutrophils. The binding of P-selectin to monocytes in the area of vascular injury may be a component of a mechanism that initiates thrombosis. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 19 |
| Volume Number | 91 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1994-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Monocytes Metabolism Platelet Membrane Glycoproteins Pharmacology Thromboplastin Cell Adhesion Molecules Cells, Cultured Dose-Response Relationship, Drug Gene Expression Drug Effects In Vitro Techniques P-Selectin RNA, Messenger Genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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