NDLI: A biomarker that identifies senescent human cells in culture and in aging skin in vivo.

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A biomarker that identifies senescent human cells in culture and in aging skin in vivo.

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A biomarker that identifies senescent human cells in culture and in aging skin in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, X. Medrano, E. E. Acosta, M. Dimri, G. P. Rubelj, I. Roskelley, C. Pereira-smith, O. Basile, G. Scott, G. Linskens, M.
Description	Author Affiliation: Dimri GP ( Department of Cell and Molecular Biology, Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.);
Abstract	Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is ample evidence that escape from senescence, or immortality, is important for malignant transformation. By contrast, the role of replicative senescence in organismic aging is controversial. Studies on cells cultured from donors of different ages, genetic backgrounds, or species suggest that senescence occurs in vivo and that organismic lifespan and cell replicative lifespan are under common genetic control. However, senescent cells cannot be distinguished from quiescent or terminally differentiated cells in tissues. Thus, evidence that senescent cells exist and accumulate with age in vivo is lacking. We show that several human cells express a beta-galactosidase, histochemically detectable at pH 6, upon senescence in culture. This marker was expressed by senescent, but not presenescent, fibroblasts and keratinocytes but was absent from quiescent fibroblasts and terminally differentiated keratinocytes. It was also absent from immortal cells but was induced by genetic manipulations that reversed immortality. In skin samples from human donors of different age, there was an age-dependent increase in this marker in dermal fibroblasts and epidermal keratinocytes. This marker provides in situ evidence that senescent cells may exist and accumulate with age in vivo.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Aging Skin Aging Skin Enzymology Beta-Galactosidase Biosynthesis Biological Markers Cell Differentiation Cell Division Cell Line Cell Transformation, Neoplastic Cells, Cultured DNA Epidermis Fibroblasts Cytology HeLa Cells Keratinocytes Pathology Skin Neoplasms Surgery Thymidine Metabolism Comparative Study Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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