NDLI: Intracellular pH in adipocytes: effects of free fatty acid diffusion across the plasma membrane, lipolytic agonists, and insulin.

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Intracellular pH in adipocytes: effects of free fatty acid diffusion across the plasma membrane, lipolytic agonists, and insulin.

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Intracellular pH in adipocytes: effects of free fatty acid diffusion across the plasma membrane, lipolytic agonists, and insulin.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Civelek, V. N. Hamilton, J. A. Kelly, K. L. Corkey, B. E. Tornheim, K.
Description	Author Affiliation: Civelek VN ( Evans Department of Medicine, Boston University Medical Center, MA 02118, USA.);
Abstract	The main function of white adipose tissue is to store nutrient energy in the form of triglycerides. The mechanism by which free fatty acids (FFA) move into and out of the adipocyte has not been resolved. We show here that changes in intracellular pH (pH1) in adipocytes correlate with the movement of FFA across cellular membranes as predicted by the Kamp and Hamilton model of passive diffusion of FFA. Exposure of fat cells to lipolytic agents or external FFA results is a rapid intracellular acidification that is reversed by metabolism of the FFA or its removal by albumin. In contrast, insulin causes an alkalinization of the cell, consistent with its main function to promote esterification. Inhibition of Na+/H+ exchange in adipocytes does not prevent the changes in pHi caused by FFA, lipolytic agents, or insulin. A fatty acid dimer, which diffuses into the cell but is not metabolized, causes an irreversible acidification. Taken together, the data suggest that changes in pHi occur in adipocytes in response to the passive diffusion of un-ionized FFA (flip-flop) into and out of the cell and in response to their metabolism and production within the cell. These changes in pHi may, in turn, modulate hormonal signaling and metabolism with significant impact on cell function.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	19
Volume Number	93
Language	English
Publisher	National Academy of Sciences
Publisher Date	1996-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Adipocytes Metabolism Fatty Acids, Nonesterified Pharmacology Hydrogen-Ion Concentration Insulin Drug Effects Amiloride Analogs & Derivatives Animals Cell Membrane Cells, Cultured Colforsin Diffusion Fluoresceins Fluorescent Dyes Isoproterenol Kinetics Lipolysis Models, Biological Norepinephrine Rats, Sprague-Dawley Serum Albumin, Bovine Sodium Time Factors Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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