NDLI: The nuclear matrix revealed by eluting chromatin from a cross-linked nucleus.

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The nuclear matrix revealed by eluting chromatin from a cross-linked nucleus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Krockmalnic, G. Nickerson, J. A. Penman, S. Wan, K. M.
Description	Author Affiliation: Nickerson JA ( Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA. Nickerson@wccf.mit.edu);
Abstract	The nucleus is an intricately structured integration of many functional domains whose complex spatial organization is maintained by a nonchromatin scaffolding, the nuclear matrix. We report here a method for preparing the nuclear matrix with improved preservation of ultrastructure. After the removal of soluble proteins, the structures of the nucleus were extensively cross-linked with formaldehyde. Surprisingly, the chromatin could be efficiently removed by DNase I digestion leaving a well preserved nuclear matrix. The nuclear matrix uncovered by this procedure consisted of highly structured fibers, connected to the nuclear lamina and built on an underlying network of branched 10-nm core filaments. The relative ease with which chromatin and the nuclear matrix could be separated despite extensive prior cross-linking suggests that there are few attachment points between the two structures other than the connections at the bases of chromatin loops. This is an important clue for understanding chromatin organization in the nucleus.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	94
Language	English
Publisher	National Academy of Sciences
Publisher Date	1997-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Chromatin Ultrastructure Histocytological Preparation Techniques Nuclear Matrix Cells, Cultured Cross-Linking Reagents DNA Drug Effects Deoxyribonuclease I Pharmacology Formaldehyde Histones Isolation & Purification RNA Ribonucleoproteins Staining And Labeling Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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