NDLI: Identification of the lactococcal exonuclease/recombinase and its modulation by the putative Chi sequence.

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Identification of the lactococcal exonuclease/recombinase and its modulation by the putative Chi sequence.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ehrlich, D. El Karoui, M. Gruss, A.
Description	Author Affiliation: el Karoui M ( Institut National de la Recherche Agronomique, Jouy en Josas, France.);
Abstract	Studies of RecBCD-Chi interactions in Escherichia coli have served as a model to understand recombination events in bacteria. However, the existence of similar interactions has not been demonstrated in bacteria unrelated to E. coli. We developed an in vivo model to examine components of dsDNA break repair in various microorganisms. Here, we identify the major exonuclease in Lactococcus lactis, a Gram-positive organism evolutionarily distant from E. coli, and provide evidence for exonuclease-Chi interactions. Insertional mutants of L. lactis, screened as exonuclease-deficient, affected a single locus and resulted in UV sensitivity and recombination deficiency. The cloned lactococcal genes (called rexAB) restored UV resistance, recombination proficiency, and the capacity to degrade linear DNA, to an E. coli recBCD mutant. In this context, DNA degradation is specifically blocked by the putative lactococcal Chi site (5'-GCGCGTG-3'), but not by the E. coli Chi (5'-GCTGGTGG-3') site. RexAB-mediated recombination was shown to be stimulated approximately 27-fold by lactococcal Chi. Our results reveal that RexAB fulfills the biological roles of RecBCD and indicate that its activity is modulated by a short DNA sequence. We speculate that exonuclease/recombinase enzymes whose activities are modulated by short DNA sequences are widespread among bacteria.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	2
Volume Number	95
Language	English
Publisher	National Academy of Sciences
Publisher Date	1998-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Exodeoxyribonucleases Metabolism Genes, Bacterial Lactococcus Lactis Enzymology Recombination, Genetic Cloning, Molecular Exodeoxyribonuclease V Genetics Molecular Sequence Data Plasmids Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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