NDLI: Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the housefly.

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Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the housefly.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Benedict, M. Q. Coates, C. J. Jasinskiene, N. Cornel, A. J. Rafferty, C. S. James, A. A. Collins, F. H.
Description	Author Affiliation: Jasinskiene N ( Department of Molecular Biology and Biochemistry, Bio Sci II, Room 3205, University of California, Irvine, CA 92697-3900, USA.);
Abstract	The mosquito Aedes aegypti is the world's most important vector of yellow fever and dengue viruses. Work is currently in progress to control the transmission of these viruses by genetically altering the capacity of wild Ae. aegypti populations to support virus replication. The germ-line transformation system reported here constitutes a major advance toward the implementation of this control strategy. A modified Hermes transposon carrying a 4.7-kb fragment of genomic DNA that includes a wild-type allele of the Drosophila melanogaster cinnabar (cn) gene was used to transform a white-eyed recipient strain of Ae. aegypti. Microinjection of preblastoderm mosquito embryos with this construct resulted in 50% of the emergent G0 adults showing some color in their eyes. Three transformed families were recovered, each resulting from an independent insertion event of the cn+-carrying transposon. The cn+ gene functioned as a semidominant transgene and segregated in Mendelian ratios. Hermes shows great promise as a vector for efficient, heritable, and stable transformation of this important mosquito vector species.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	7
Volume Number	95
Language	English
Publisher	National Academy of Sciences
Publisher Date	1998-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Aedes Genetics DNA Transposable Elements Houseflies Insect Vectors Receptors, Lymphocyte Homing Yellow Fever Virus Physiology Virology Animals Gene Transfer Techniques Mosquito Control Virus Replication Yellow Fever Transmission Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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