NDLI: How Cro and lambda-repressor distinguish between operators: the structural basis underlying a genetic switch.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1998 Volume: 95 Issue: 26

Year: 1998 Volume: 95 Issue: 25

Year: 1998 Volume: 95 Issue: 24

Year: 1998 Volume: 95 Issue: 23

Year: 1998 Volume: 95 Issue: 22

Year: 1998 Volume: 95 Issue: 21

Year: 1998 Volume: 95 Issue: 20

Year: 1998 Volume: 95 Issue: 19

Year: 1998 Volume: 95 Issue: 18

Year: 1998 Volume: 95 Issue: 17

Year: 1998 Volume: 95 Issue: 16

Year: 1998 Volume: 95 Issue: 15

Year: 1998 Volume: 95 Issue: 14

Year: 1998 Volume: 95 Issue: 13

Year: 1998 Volume: 95 Issue: 12

Year: 1998 Volume: 95 Issue: 11

Year: 1998 Volume: 95 Issue: 10

Year: 1998 Volume: 95 Issue: 9

Year: 1998 Volume: 95 Issue: 8

Year: 1998 Volume: 95 Issue: 7

Recent excitement regarding metallothionein.

Depudecin makes a debut.

Assaying for peptides in individual Aplysia neurons with mass spectrometry.

To muster a cluster: anchoring neurotransmitter receptors at synapses.

Visual scenes and cortical neurons: what you see is what you get.

Is sex better? Parasites say 'no'.

An important step forward in the genetic manipulation of mosquito vectors of human disease.

A gravitational diffusion model without dark matter.

Depudecin induces morphological reversion of transformed fibroblasts via the inhibition of histone deacetylase.

Creatine kinase: essential arginine residues at the nucleotide binding site identified by chemical modification and high-resolution tandem mass spectrometry.

Atmospheric weathering and silica-coated feldspar: analogy with zeolite molecular sieves, granite weathering, soil formation, ornamental slabs, and ceramics.

Biochemical evolution. I. Polymerization On internal, organophilic silica surfaces of dealuminated zeolites and feldspars.

The hochschild cohomology problem for von neumann algebras.

Anticancer action of cubé insecticide: correlation for rotenoid constituents between inhibition of NADH:ubiquinone oxidoreductase and induced ornithine decarboxylase activities.

Plasmodium gallinaceum preferentially invades vesicular ATPase-expressing cells in Aedes aegypti midgut.

High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping.

Linker histone protects linker DNA on only one side of the core particle and in a sequence-dependent manner.

A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides.

Crystal structure of the catalytic domain of human tumor necrosis factor-alpha-converting enzyme.

Crystal structure of MTCP-1: implications for role of TCL-1 and MTCP-1 in T cell malignancies.

HIV-1 Vpr interacts with a human 34-kDa mov34 homologue, a cellular factor linked to the G2/M phase transition of the mammalian cell cycle.

The transmembrane domain in viral fusion: essential role for a conserved glycine residue in vesicular stomatitis virus G protein.

How Cro and lambda-repressor distinguish between operators: the structural basis underlying a genetic switch.

The crystal structure of a 3D domain-swapped dimer of RNase A at a 2.1-A resolution.

Rack-induced metal binding vs. flexibility: Met121His azurin crystal structures at different pH.

$IPF_{2α}-I:$ An index of lipid peroxidation in humans

Transcriptional sequencing: A method for DNA sequencing using RNA polymerase.

Myeloid-specific transcriptional activation by murine myeloid zinc-finger protein 2.

Derepression of the C/EBPalpha gene during adipogenesis: identification of AP-2alpha as a repressor.

Structure and mechanism of a proline-specific aminopeptidase from Escherichia coli.

Thiolate ligands in metallothionein confer redox activity on zinc clusters.

The glutathione redox couple modulates zinc transfer from metallothionein to zinc-depleted sorbitol dehydrogenase.

Control of zinc transfer between thionein, metallothionein, and zinc proteins.

In vitro disassembly and reassembly of an ABC transporter, the histidine permease.

Gene rearrangement attenuates expression and lethality of a nonsegmented negative strand RNA virus.

Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways.

Membrane physical state controls the signaling mechanism of the heat shock response in Synechocystis PCC 6803: Identification of hsp17 as a “fluidity gene”

A role for histone deacetylase activity in HDAC1-mediated transcriptional repression.

23S rRNA positions essential for tRNA binding in ribosomal functional sites.

The domain organization of NaeI endonuclease: separation of binding and catalysis.

Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1.

Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein.

Stimulation of p70S6 kinase via a growth hormone-controlled phosphatidylinositol 3-kinase pathway leads to the activation of a PDE4A cyclic AMP-specific phosphodiesterase in 3T3-F442A preadipocytes.

Epoxidation of olefins by cytochrome P450: Evidence from site-specific mutagenesis for hydroperoxo-iron as an electrophilic oxidant

ESA1 is a histone acetyltransferase that is essential for growth in yeast.

Diffusion of peroxynitrite across erythrocyte membranes.

Global flexibility of tertiary structure in RNA: yeast tRNAPhe as a model system.

Oxidized, deaminated cytosines are a source of C --> T transitions in vivo.

Structure-based prediction of the stability of transmembrane helix-helix interactions: the sequence dependence of glycophorin A dimerization.

Rational design of a triple helix-specific intercalating ligand.

Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL.

Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells.

Impact of oncogenes in tumor angiogenesis: mutant K-ras up-regulation of vascular endothelial growth factor/vascular permeability factor is necessary, but not sufficient for tumorigenicity of human colorectal carcinoma cells.

Identification of cytokeratin 1 as a binding protein and presentation receptor for kininogens on endothelial cells.

The expression of matrix metalloproteinase 9 is enhanced by Epstein-Barr virus latent membrane protein 1.

Regulation of the Escherichia coli water channel gene aqpZ.

Functional association between Arf and RalA in active phospholipase D complex.

Cloned mammalian neutral sphingomyelinase: functions in sphingolipid signaling?

Etk/Bmx, a tyrosine kinase with a pleckstrin-homology domain, is an effector of phosphatidylinositol 3'-kinase and is involved in interleukin 6-induced neuroendocrine differentiation of prostate cancer cells.

Packaging of intron-containing genes into retrovirus vectors by alphavirus vectors.

Synthetic activation of caspases: Artificial death switches

Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (alpha,beta)methylenediphosphonate.

The tumor suppressor SMAD4/DPC4 is essential for epiblast proliferation and mesoderm induction in mice.

Over one-half billion years of head conservation? Expression of an ems class gene in Hydractinia symbiolongicarpus (Cnidaria: Hydrozoa).

Production of medakafish chimeras from a stable embryonic stem cell line.

Competition among body parts in the development and evolution of insect morphology.

A protein with protective properties against the cellular defense reactions in insects.

Populations can persist in an environment consisting of sink habitats only.

Marine latitudinal diversity gradients: tests of causal hypotheses.

Codon reassignment and amino acid composition in hemichordate mitochondria.

Positive Darwinian selection after gene duplication in primate ribonuclease genes.

Major histocompatibility complex variation associated with juvenile survival and parasite resistance in a large unmanaged ungulate population.

Strand compositional asymmetry in bacterial and large viral genomes.

Archaeal translation initiation revisited: the initiation factor 2 and eukaryotic initiation factor 2B alpha-beta-delta subunit families.

Data transferability from model organisms to human beings: insights from the functional genomics of the flightless region of Drosophila.

Developmental rescue of Drosophila cephalic defects by the human Otx genes.

Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the housefly.

Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti.

Parallel analysis of genetic selections using whole genome oligonucleotide arrays.

Overlapping roles and asymmetrical cross-regulation of the USF proteins in mice.

Rapid isolation of cDNA by hybridization.

Unusual features of the Drosophila melanogaster telomere transposable element HeT-A are conserved in Drosophila yakuba telomere elements.

Probing the genetic population structure of Trypanosoma cruzi with polymorphic microsatellites.

Molecular anatomy of chromosome 7q deletions in myeloid neoplasms: evidence for multiple critical loci.

A third distinct tumor necrosis factor receptor of orthopoxviruses.

NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176.

Natural killer activating receptors trigger interferon gamma secretion from T cells and natural killer cells.

The role of peptides in thymic positive selection of class II major histocompatibility complex-restricted T cells.

Cytokine-induced survival of activated T cells in vitro and in vivo.

High affinity cross-reacting mAb generated by minimal mimicry: implications for the pathogenesis of anti-nuclear autoantibodies and immunosuppression.

Antibody-mediated targeting of CD45 isoforms: a novel immunotherapeutic strategy.

The Tpl-2 protooncoprotein activates the nuclear factor of activated T cells and induces interleukin 2 expression in T cell lines.

T cell epitopes of insulin defined in HLA-DR4 transgenic mice are derived from preproinsulin and proinsulin.

A conserved sequence block in murine and human T cell receptor (TCR) Jalpha region is a composite element that enhances TCR alpha enhancer activity and binds multiple nuclear factors.

Recruitment of SH2-containing protein tyrosine phosphatase SHP-1 to the interleukin 2 receptor; loss of SHP-1 expression in human T-lymphotropic virus type I-transformed T cells

Interferon alpha and Tat involvement in the immunosuppression of uninfected T cells and C-C chemokine decline in AIDS.

C-C chemokines, pivotal in protection against HIV type 1 infection.

An X chromosome gene regulates hematopoietic stem cell kinetics.

Protection against peroxynitrite-induced fibroblast injury and arthritis development by inhibition of poly(ADP-ribose) synthase.

Transcriptional map of 170-kb region at chromosome 11p15.5: identification and mutational analysis of the BWR1A gene reveals the presence of mutations in tumor samples.

Analysis of ClC-2 channels as an alternative pathway for chloride conduction in cystic fibrosis airway cells.

Deregulated expression of TCL1 causes T cell leukemia in mice.

The T cell leukemia LIM protein Lmo2 is necessary for adult mouse hematopoiesis.

Taxane-mediated gene induction is independent of microtubule stabilization: induction of transcription regulators and enzymes that modulate inflammation and apoptosis.

Identification and isolation of candidate human keratinocyte stem cells based on cell surface phenotype.

Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats--similarities to astrocyte grafts.

Budesonide epimer R or dexamethasone selectively inhibit platelet-activating factor-induced or interleukin 1beta-induced DNA binding activity of cis-acting transcription factors and cyclooxygenase-2 gene expression in human epidermal keratinocytes.

Directed changes in the number of double-stranded RNA genomic segments in bacteriophage phi6.

Epithelial attachment alters the outcome of Helicobacter pylori infection.

Herpes simplex virus 1 induces and blocks apoptosis at multiple steps during infection and protects cells from exogenous inducers in a cell-type-dependent manner.

Cytomegalovirus remains latent in a common precursor of dendritic and myeloid cells.

'Black holes' and bacterial pathogenicity: a large genomic deletion that enhances the virulence of Shigella spp. and enteroinvasive Escherichia coli.

Virulence of antibiotic-resistant Salmonella typhimurium.

Recombinant viruses expressing a human malaria antigen can elicit potentially protective immune $CD8^{+}$ responses in mice

staggerer phenotype in retinoid-related orphan receptor alpha-deficient mice.

Neuronal polarity: essential role of protein-lipid complexes in axonal sorting.

Proteolytic processing of the Aplysia egg-laying hormone prohormone.

Prolonged delivery of brain-derived neurotrophic factor by adenovirus-infected Müller cells temporarily rescues injured retinal ganglion cells.

The small GTP-binding protein Cdc42 is required for nerve growth factor withdrawal-induced neuronal death.

Expression of monocarboxylate transporter mRNAs in mouse brain: support for a distinct role of lactate as an energy substrate for the neonatal vs. adult brain.

A tripotential glial precursor cell is present in the developing spinal cord.

The distribution of oriented contours in the real world.

Mechanism of nicotinic acetylcholine receptor cluster formation by rapsyn.

Nurr1 is essential for the induction of the dopaminergic phenotype and the survival of ventral mesencephalic late dopaminergic precursor neurons.

Cloning and functional expression of alternative spliced variants of the rho1 gamma-aminobutyrate receptor.

Developmental patterns in the cytoarchitecture of the human cerebral cortex from birth to 6 years examined by correspondence analysis.

Profound neuronal plasticity in response to inactivation of the dopamine transporter.

Decay of prepulse facilitation of N type calcium channels during G protein inhibition is consistent with binding of a single Gbeta subunit.

Calcimimetics with potent and selective activity on the parathyroid calcium receptor

The yeast CLC chloride channel functions in cation homeostasis

Seminal fluid regulation of female sexual attractiveness in Drosophila melanogaster.

Activity patterns of neurosecretory cells releasing pheromonotropic neuropeptides in the moth Bombyx mori.

Diet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice.

Stress facilitates classical conditioning in males, but impairs classical conditioning in females through activational effects of ovarian hormones.

The Black-Scholes pricing formula in the quantum context.

Year: 1998 Volume: 95 Issue: 6

Year: 1998 Volume: 95 Issue: 5

Year: 1998 Volume: 95 Issue: 4

Year: 1998 Volume: 95 Issue: 3

Year: 1998 Volume: 95 Issue: 2

Year: 1998 Volume: 95 Issue: 1

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

How Cro and lambda-repressor distinguish between operators: the structural basis underlying a genetic switch.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Albright, R. A. Matthews, B. W.
Description	Author Affiliation: Albright RA ( Institute of Molecular Biology, Howard Hughes Medical Institute and Department of Physics, University of Oregon, Eugene, OR 97403, USA.);
Abstract	Knowledge of the three-dimensional structures of the lambda-Cro and lambda-repressor proteins in complex with DNA has made it possible to evaluate how these proteins discriminate between different operators in phage lambda. As anticipated in previous studies, the helix-turn-helix units of the respective proteins bind in very different alignments. In Cro the recognition helices are 29 A apart and are tilted by 55 degrees with respect to each other, but bind parallel to the major groove of the DNA. In lambda-repressor [Beamer, L. J. & Pabo, C. O. (1992) J. Mol. Biol. 227, 177-196] the helices are 34 A apart and are essentially parallel to each other, but are inclined to the major grooves. The DNA is much more bent when bound by Cro than in the case with lambda-repressor. The first two amino acids of the recognition helices of the two proteins, Gln-27 and Ser-28 in Cro, and Gln-44 and Ser-45 in lambda-repressor, make very similar interactions with the invariant bps 2 and 4. There are also analogous contacts between the thymine of bp 5 and, respectively, the backbone of Ala-29 of Cro and the backbone of Gly-46 of lambda-repressor. Otherwise, however, unrelated parts of the two proteins are used in sequence-specific recognition. It appears that similar contacts to the invariant or almost invariant bps (especially 2 and 4) are used by both Cro and lambda-repressor to differentiate the operator sites as a group from other sites on the DNA. The discrimination of Cro and lambda-repressor between their different operators is more subtle and seems to be achieved primarily through differences in van der Waals contacts at bp 3', together with weaker, less direct effects at bps 5' and 8', all in the nonconsensus half of the operators. The results provide further support for the idea that there is no simple code for DNA-protein recognition.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	7
Volume Number	95
Language	English
Publisher	National Academy of Sciences
Publisher Date	1998-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacteriophage Lambda Chemistry DNA, Viral DNA-Binding Proteins Repressor Proteins Genetics Binding Sites Molecular Sequence Data Operator Regions, Genetic Protein Binding Protein Conformation Structure-Activity Relationship Viral Proteins Viral Regulatory And Accessory Proteins Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in