NDLI: Calnuc, an EF-Hand $Ca^{2+}$ binding protein, specifically interacts with the C-terminal α5-helix of Gαi3

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 2000 Volume: 97 Issue: 26

Year: 2000 Volume: 97 Issue: 25

Year: 2000 Volume: 97 Issue: 24

Year: 2000 Volume: 97 Issue: 23

Year: 2000 Volume: 97 Issue: 22

Year: 2000 Volume: 97 Issue: 21

Year: 2000 Volume: 97 Issue: 20

Year: 2000 Volume: 97 Issue: 19

Year: 2000 Volume: 97 Issue: 18

Year: 2000 Volume: 97 Issue: 17

Year: 2000 Volume: 97 Issue: 16

Year: 2000 Volume: 97 Issue: 15

Year: 2000 Volume: 97 Issue: 14

Year: 2000 Volume: 97 Issue: 13

Year: 2000 Volume: 97 Issue: 12

Year: 2000 Volume: 97 Issue: 11

Year: 2000 Volume: 97 Issue: 10

Year: 2000 Volume: 97 Issue: 9

Year: 2000 Volume: 97 Issue: 8

Year: 2000 Volume: 97 Issue: 7

Year: 2000 Volume: 97 Issue: 6

Year: 2000 Volume: 97 Issue: 5

Year: 2000 Volume: 97 Issue: 4

Year: 2000 Volume: 97 Issue: 3

Year: 2000 Volume: 97 Issue: 2

PNAS Early Edition.

Lysozyme among the Lilliputians.

Bax-induced apoptotic cell death.

Antitumorigenic actions of growth hormone-releasing hormone antagonists.

Another hormone-sensitive triglyceride lipase in fat cells?

Chemistry and biology.

Quantum groups with invariant integrals.

The operator algebra approach to quantum groups.

Architecture with designer atoms: simple theoretical considerations.

A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes.

Highly mutagenic replication by DNA polymerase V (UmuC) provides a mechanistic basis for SOS untargeted mutagenesis.

Acceleration of oligomerization, not fibrillization, is a shared property of both alpha-synuclein mutations linked to early-onset Parkinson's disease: implications for pathogenesis and therapy.

Proapoptotic BH3-only Bcl-2 family members induce cytochrome c release, but not mitochondrial membrane potential loss, and do not directly modulate voltage-dependent anion channel activity.

Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli.

The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium.

Evidence for a linear search in bimolecular 3' splice site AG selection.

NifS-directed assembly of a transient [2Fe-2S] cluster within the NifU protein.

In vivo selection of functional ribosomes with variations in the rRNA-binding site of Escherichia coli ribosomal protein S8: evolutionary implications.

Modifications of proteins by polyunsaturated fatty acid peroxidation products.

Direct localization of a beta-subunit domain on the three-dimensional structure of Escherichia coli RNA polymerase.

Specific chemical and structural damage to proteins produced by synchrotron radiation.

Sequence-specific binding of counterions to B-DNA.

Landscape approaches for determining the ensemble of folding transition states: success and failure hinge on the degree of frustration.

Mechanism of the single-headed processivity: diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin.

RNA folding energy landscapes.

CopA: An Escherichia coli Cu(I)-translocating P-type ATPase.

Regulation of expanded polyglutamine protein aggregation and nuclear localization by the glucocorticoid receptor.

Drob-1, a Drosophila member of the Bcl-2/CED-9 family that promotes cell death

The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate.

Calnuc, an EF-Hand $Ca^{2+}$ binding protein, specifically interacts with the C-terminal α5-helix of Gαi3

A Rab11-containing rapidly recycling compartment in macrophages that promotes phagocytosis.

A method for detecting abasic sites in living cells: age-dependent changes in base excision repair.

Position-dependent linkages of fibronectin- integrin-cytoskeleton.

Heritability of fitness in a wild mammal population.

Functional evolution of the Ultrabithorax protein.

Distinct genetic profiles in colorectal tumors with or without the CpG island methylator phenotype.

Heterochromatic deposition of centromeric histone H3-like proteins

Double trans-chromosomic mice: maintenance of two individual human chromosome fragments containing Ig heavy and kappa loci and expression of fully human antibodies.

Plasmid copy-number control and better-than-random segregation genes of pSM19035 share a common regulator.

IL-18 regulates IL-1beta-dependent hepatic melanoma metastasis via vascular cell adhesion molecule-1.

Involvement of decidual Valpha14 NKT cells in abortion.

Enzymatic reduction of disulfide bonds in lysosomes: characterization of a gamma-interferon-inducible lysosomal thiol reductase (GILT).

Pathogen-specific loss of host resistance in mice lacking the IFN-gamma-inducible gene IGTP.

T cell receptor interactions with class I heavy-chain influence T cell selection.

Prevention of passively transferred experimental autoimmune myasthenia gravis by a phage library-derived cyclic peptide.

Anti-IL-9 vaccination prevents worm expulsion and blood eosinophilia in Trichuris muris-infected mice.

Cloning and characterization of IL-17B and IL-17C, two new members of the IL-17 cytokine family.

Benzo[a]pyrene carcinogenicity is lost in mice lacking the aryl hydrocarbon receptor.

Drug target validation: lethal infection blocked by inducible peptide.

Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy, but not in obesity.

Adenoviral gene transfer of SERCA2a improves left-ventricular function in aortic-banded rats in transition to heart failure

Intestinal trefoil factor confers colonic epithelial resistance to apoptosis.

Functional deletion of the CCR5 receptor by intracellular immunization produces cells that are refractory to CCR5-dependent HIV-1 infection and cell fusion.

Cationic microparticles: A potent delivery system for DNA vaccines.

ABCG1 (ABC8), the human homolog of the Drosophila white gene, is a regulator of macrophage cholesterol and phospholipid transport.

An in vivo approach showing the chemotactic activity of leukotriene B(4) in acute renal ischemic-reperfusion injury.

Stability of cytotoxic luteinizing hormone-releasing hormone conjugate (AN-152) containing doxorubicin 14-O-hemiglutarate in mouse and human serum in vitro: implications for the design of preclinical studies.

Extracellular protein kinase A as a cancer biomarker: its expression by tumor cells and reversal by a myristate-lacking Calpha and RIIbeta subunit overexpression.

Aortic wall damage in mice unable to synthesize ascorbic acid.

Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization.

Specific chromosome 1 breaks induced by human cytomegalovirus.

Genetic analysis of a bacterial genetic exchange element: the gene transfer agent of Rhodobacter capsulatus.

Loss of BETA2/NeuroD leads to malformation of the dentate gyrus and epilepsy.

ATM is a cytoplasmic protein in mouse brain required to prevent lysosomal accumulation.

Direct evidence for local oscillatory current sources and intracortical phase gradients in turtle visual cortex.

Kinetics of exocytosis and endocytosis at the cochlear inner hair cell afferent synapse of the mouse

Atm and Bax cooperate in ionizing radiation-induced apoptosis in the central nervous system.

Constraining the subunit order of rod cyclic nucleotide-gated channels reveals a diagonal arrangement of like subunits.

Dynamics of dendritic calcium transients evoked by quantal release at excitatory hippocampal synapses.

Effect of G protein heterotrimer composition on coupling of neurotransmitter receptors to N-type $Ca^{2+}$ channel modulation in sympathetic neurons

Temporal dynamics of cortical representation for action.

Theta oscillations index human hippocampal activation during a working memory task.

Biochemically based design of cyclooxygenase-2 (COX-2) inhibitors: facile conversion of nonsteroidal antiinflammatory drugs to potent and highly selective COX-2 inhibitors.

Overexpression of angiotensin II type I receptor in cardiomyocytes induces cardiac hypertrophy and remodeling.

Modulation of nucleotide sensitivity of ATP-sensitive potassium channels by phosphatidylinositol-4-phosphate 5-kinase

Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis.

An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration.

Year: 2000 Volume: 97 Issue: 1

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Calnuc, an EF-Hand $Ca^{2+}$ binding protein, specifically interacts with the C-terminal α5-helix of Gαi3

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lin, P. Weiss, T. Farquhar, M. G. Fischer, T.
Description	Author Affiliation: Lin P ( Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093-0651, USA.);
Abstract	Calnuc (nucleobindin) was previously shown to be present both in the cytosol and in the Golgi and to be the major Golgi Ca(2+) binding protein. In this study we verified the existence of the cytosolic pool of calnuc and investigated its interaction with G(alpha)i3. Cytosolic calnuc was released by mild digitonin permeabilization. In pulse-chase experiments, the two pools of calnuc had different mobilities, suggesting different posttranslational modifications. That calnuc interacts with G(alpha)i3 in vivo was verified by the finding that G(alpha)i3 could be crosslinked intracellularly to calnuc and co-immunoprecipitated from NIH 3T3 cells stably overexpressing either activated (Q204L) or inactivated (G203A) G(alpha)i3. Binding was Ca(2+) and Mg(2+)-dependent. Calnuc and G(alpha)i3-GFP codistributed primarily in the Golgi region. By yeast two-hybrid analysis, the binding site on G(alpha)i3 for calnuc was mapped to the C-terminal region because removal of the last 12 amino acids (but not 11) abolished the interaction. Peptide competition indicated that calnuc, with its coiled-coil domain constituted by the two EF-hands, binds to G(alpha)i3's C-terminal alpha5-helix. These results demonstrate that calnuc may play an important role in G protein- and Ca(2+)-regulated signal transduction events.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	2
Volume Number	97
Language	English
Publisher	National Academy of Sciences
Publisher Date	2000-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Calcium-Binding Proteins Metabolism DNA-Binding Proteins GTP-Binding Protein Alpha Subunits, Gi-Go Growth Substances 3T3 Cells Amino Acid Sequence Animals Binding Sites CHO Cells Calcium Pharmacology Cell Line Cell Membrane Cricetinae Cytosol Fluorescent Antibody Technique Chemistry Genetics Golgi Apparatus Green Fluorescent Proteins Intracellular Membranes Luminescent Proteins Magnesium Mice Molecular Sequence Data Nerve Tissue Proteins Protein Binding Drug Effects Recombinant Fusion Proteins Sequence Deletion Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in