NDLI: Mechanism of the single-headed processivity: diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin.

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Mechanism of the single-headed processivity: diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hirokawa, N. Okada, Y.
Description	Author Affiliation: Okada Y ( Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.);
Abstract	A motor-domain construct of KIF1A, a single-headed kinesin superfamily protein, was demonstrated to take more than 600 steps before detaching from a microtubule. However, its molecular mechanism remained unclear. Here we demonstrate the nucleotide-dependent binding between the lysine-rich, highly positively charged loop 12 of the KIF1A motor domain (K-loop) and the glutamate-rich, highly negatively charged C-terminal region of tubulin (E-hook). This binding did not contribute in the strong binding state but only in the weak binding state. This binding was demonstrated to be essential for the single-headed processivity by functioning as the anchor for the one-dimensional simple Brownian movement in the weak binding state. This Brownian movement will allow the small KIF1A motor domain to span the distance between the binding sites on microtubule and also will give the diffusive nature to the movement of single KIF1A molecules. These observations quantitatively fitted well to the predictions made from our Brownian motor model on the mechanism of the single-headed processive movement.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	2
Volume Number	97
Language	English
Publisher	National Academy of Sciences
Publisher Date	2000-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Kinesin Metabolism Tubulin Adenosine Diphosphate Pharmacology Adenosine Triphosphate Amino Acid Sequence Binding Sites Genetics Chemistry Kinetics Lysine Microtubules Models, Biological Molecular Sequence Data Mutation Protein Binding Drug Effects Sequence Homology, Amino Acid Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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