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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Knuth, A. Nagata, Y. Lee, S. Y. Karbach, J. Old, L. J. Dunbar, P. R. Jungbluth, A. Jäger, D. Wada, H. Gnjatic, S. Stockert, E. Cerundolo, V. Arand, M. Ritter, G. Chen, Y. T. Dupont, B. Jäger, E. |
| Description | Author Affiliation: Jäger E ( II. Medizinische Klinik, Hämatologie-Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany. 100333.1434@compuserve.com); |
| Abstract | NY-ESO-1, a member of the cancer-testis family of antigens, is expressed in a subset of a broad range of different human tumor types. Patients with advanced NY-ESO-1-expressing tumors frequently develop humoral immunity to NY-ESO-1, and three HLA A2-restricted peptides were defined previously as targets for cytotoxic CD8(+) T cells in a melanoma patient with NY-ESO-1 antibody. The objectives of the present study were (i) to develop enzyme-linked immunospot (ELISPOT) and tetramer assays to measure CD8(+) T cell responses to NY-ESO-1, (ii) to determine the frequency of CD8(+) T cell responses to NY-ESO-1 in a series of HLA-A2 patients with NY-ESO-1 expressing tumors, (iii) to determine the relation between CD8(+) T cell and humoral immune responses to NY-ESO-1, and (iv) to compare results of NY-ESO-1 ELISPOT assays performed independently in two laboratories with T cells from the same patients. NY-ESO-1 ELISPOT and tetramer assays with excellent sensitivity, specificity, and reproducibility have been developed and found to correlate with cytotoxicity assays. CD8(+) T cell responses to HLA-A2-restricted NY-ESO-1 peptides were detected in 10 of 11 patients with NY-ESO-1 antibody, but not in patients lacking antibody or in patients with NY-ESO-1-negative tumors. The results of ELISPOT assays were concordant in the two laboratories, providing the basis for standardized monitoring of T cell responses in patients receiving NY-ESO-1 vaccines. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 9 |
| Volume Number | 97 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2000-05-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Antigens, Neoplasm Immunology CD8-Positive T-Lymphocytes Melanoma Membrane Proteins Proteins Antibody Formation Drug Effects Cells, Cultured Cytotoxicity, Immunologic Enzyme-Linked Immunosorbent Assay HLA-A2 Antigen Chemistry Histocompatibility Testing Immunity, Cellular Peptide Fragments Pharmacology Genetics RNA, Messenger Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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