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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pinaud, F. Lacoste, T. D. Chemla, D. S. Alivisatos, A. P. Michalet, X. Weiss, S. |
| Description | Author Affiliation: Lacoste TD ( Material Sciences and Physical Biosciences Divisions, Lawrence Berkeley National Laboratory, CA 94720, USA.); |
| Abstract | An optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described in this paper. It relies on the use of two unique families of fluorophores, namely energy-transfer fluorescent beads (TransFluoSpheres) and semiconductor nanocrystal quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A multicolor sample-scanning confocal microscope was constructed that allows one to image each fluorescent light emitter, free of chromatic aberrations, by scanning the sample with nanometer scale steps with a piezo-scanner. The resulting spots are accurately localized by fitting them to the known shape of the excitation point-spread function of the microscope. We present results of two-dimensional colocalization of TransFluoSpheres (40 nm in diameter) and of nanocrystals (3-10 nm in diameter) and demonstrate distance-measurement accuracy of better than 10 nm using conventional far-field optics. This ruler bridges the gap between fluorescence resonance energy transfer, near- and far-field imaging, spanning a range of a few nanometers to tens of micrometers. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 17 |
| Volume Number | 97 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2000-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Fluorescent Dyes Metabolism Molecular Probe Techniques Instrumentation Energy Transfer Fluorescence Microscopy, Confocal Sensitivity And Specificity Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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