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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ho, N. T. Lukin, J. A. Ho, C. Simplaceanu, V. Zou, M. |
| Description | Author Affiliation: Lukin JA ( Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213-2683, USA.); |
| Abstract | Compared with free heme, the proteins hemoglobin (Hb) and myoglobin (Mb) exhibit greatly enhanced affinity for oxygen relative to carbon monoxide. This physiologically vital property has been attributed to either steric hindrance of CO or stabilization of O(2) binding by a hydrogen bond with the distal histidine. We report here the first direct evidence of such a hydrogen bond in both alpha- and beta-chains of oxyhemoglobin, as revealed by heteronuclear NMR spectra of chain-selectively labeled samples. Using these spectra, we have assigned the imidazole ring (1)H and (15)N chemical shifts of the proximal and distal histidines in both carbonmonoxy- and oxy-Hb. Because of their proximity to the heme, these chemical shifts are extremely sensitive to the heme pocket conformation. Comparison of the measured chemical shifts with values predicted from x-ray structures suggests differences between the solution and crystal structures of oxy-Hb. The chemical shift discrepancies could be accounted for by very small displacements of the proximal and distal histidines. This suggests that NMR could be used to obtain very high-resolution heme pocket structures of Hb, Mb, and other heme proteins. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 19 |
| Volume Number | 97 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2000-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Histidine Chemistry Oxygen Oxyhemoglobins Crystallization Hydrogen Bonding Magnetic Resonance Spectroscopy Models, Molecular Protein Conformation Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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