NDLI: Multiplex allele-specific target amplification based on PCR suppression.

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Multiplex allele-specific target amplification based on PCR suppression.

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Multiplex allele-specific target amplification based on PCR suppression.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, L. Englert, D. Woodward, K. Broude, N. E. Cantor, C. R.
Description	Author Affiliation: Broude NE ( Center for Advanced Biotechnology and Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA. nebroude@hotmail.com);
Abstract	We have developed a strategy for multiplex PCR based on PCR suppression. PCR suppression allows DNA target amplification with only one sequence-specific primer per target and a second primer that is common for all targets. Therefore, an n-plex PCR would require only n + 1 primers. We have demonstrated uniform, efficient amplification of targeted sequences in 14-plex PCR. The high specificity of suppression PCR also provides multiplexed amplification with allele specificity. Multiplexed PCR was used to develop assays for genotyping DNA samples from cystic fibrosis-affected individuals. The new approach greatly simplifies primer design, significantly increases the PCR multiplexing level, and decreases the overall primer cost. In addition, this assay is more readily amenable to automation and is therefore suitable for high-throughput genetic diagnostics.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	98
Language	English
Publisher	National Academy of Sciences
Publisher Date	2001-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Alleles Gene Targeting Nucleic Acid Amplification Techniques Polymerase Chain Reaction Automation Chromosomes, Human, Pair 7 Genetics Cystic Fibrosis Cystic Fibrosis Transmembrane Conductance Regulator DNA Mutational Analysis DNA Primers DNA-Directed DNA Polymerase Metabolism Genetic Testing Genotype Oligodeoxyribonucleotides Substrate Specificity Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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