NDLI: 2-Aminopurine fluorescence quenching and lifetimes: role of base stacking.

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2-Aminopurine fluorescence quenching and lifetimes: role of base stacking.

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2-Aminopurine fluorescence quenching and lifetimes: role of base stacking.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jean, J. M. Hall, K. B.
Description	Author Affiliation: Jean JM ( Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.);
Abstract	2-Aminopurine (2AP) is a fluorescent analog of guanosine and adenosine and has been used to probe nucleic acid structure and dynamics. Its spectral features in nucleic acids have been interpreted phenomenologically, in the absence of a rigorous electronic description of the context-dependence of 2AP fluorescence. Now, by using time-dependent density functional theory, we describe the excited-state properties of 2AP in a B-form dinucleotide stacked with guanosine, adenosine, cytosine, or thymine. Calculations predict that 2AP fluorescence is quenched statically when stacked with purines, because of mixing of the molecular orbitals in the ground state. In contrast, quenching is predicted to be dynamic when 2AP is stacked with pyrimidines, because of formation of a low-lying dark excited state. The different quenching mechanisms will result in different experimentally measured fluorescence lifetimes and quantum yields.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	98
Language	English
Publisher	National Academy of Sciences
Publisher Date	2001-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	2-Aminopurine Chemistry DNA Fluorescence Pyrimidine Dimers Metabolism Adenosine Analogs & Derivatives Algorithms Base Pairing Computer Simulation Guanosine Hydrogen Bonding Kinetics Models, Molecular Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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