NDLI: A spliceosomal intron in Giardia lamblia.

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A spliceosomal intron in Giardia lamblia.

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A spliceosomal intron in Giardia lamblia.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Morrison, Hilary G. Samuelson, John Mcarthur, Andrew G. Nixon, Julie E. J. Wang, Amy Sogin, Mitchell L. Loftus, Brendan J.
Description	Author Affiliation: Nixon JE ( Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115-6018, USA.);
Abstract	Short introns occur in numerous protist lineages, but there are no reports of intervening sequences in the protists Giardia lamblia and Trichomonas vaginalis, which may represent the deepest known branches in the eukaryotic line of descent. We have discovered a 35-bp spliceosomal intron in a gene encoding a putative [2Fe-2S] ferredoxin of G. lamblia. The Giardia intron contains a canonical splice site at its 3' end (AG), a noncanonical splice site at its 5' end (CT), and a branch point sequence that fits the yeast consensus sequence of TACTAAC except for the first nucleotide (AACTAAC). We have also identified several G. lamblia genes with spliceosomal peptides, including homologues of eukaryote-specific spliceosomal peptides (Prp8 and Prp11), several DExH-box RNA-helicases that have homologues in eubacteria, but serve essential functions in the splicing of introns in eukaryotes, and 11 predicted archaebacteria-like Sm and like-Sm core peptides, which coat small nuclear RNAs. Phylogenetic analyses show the Giardia Sm core peptides are the products of multiple, ancestral gene duplications followed by divergence, but they retain strong similarity to Sm and like-Sm peptides of other eukaryotes. Although we have documented only a single intron in Giardia, it likely has other introns and fully functional, spliceosomal machinery. If introns were added during eukaryotic evolution (the introns-late hypothesis), then these results push back the date of this event before the branching of G. lamblia.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	6
Volume Number	99
Language	English
Publisher	National Academy of Sciences
Publisher Date	2002-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Evolution, Molecular Ferredoxins Genetics Giardia Lamblia Introns RNA Splicing Spliceosomes Metabolism Amino Acid Sequence Animals Conserved Sequence Genes, Protozoan Genome, Protozoan Enzymology Molecular Sequence Data Peptides Chemistry Phylogeny Protozoan Proteins RNA Splice Sites Sequence Alignment Species Specificity Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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