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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Greenbaum, Nancy L. Newby, Meredith I. |
| Description | Author Affiliation: Newby MI ( Department of Chemistry and Biochemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306-4390, USA.); |
| Abstract | The inherent chemical properties of RNA molecules are expanded by posttranscriptional modification of specific nucleotides. Pseudouridine (psi), the most abundant of the modified bases, features an additional imino group, NH1, as compared with uridine. When psi forms a Watson-Crick base pair with adenine in an RNA helix, NH1 is positioned within the major groove. The presence of psi often increases thermal stability of the helix or loop in which it is found [Hall, K. B. & McLaughlin, L. (1992) Nucleic Acids Res. 20, 1883-1889]. X-ray crystal structures of transfer RNAs [e.g., Arnez, J. & Steitz, T. (1994) Biochemistry 33, 7560-7567] have depicted water molecules bridging psiNH1 groups and nearby phosphate oxygen atoms, but direct evidence for this interaction in solution has not been acquired. Toward this end, we have used a rotating-frame Overhauser effect spectroscopy-type NMR pulse sequence with a CLEAN chemical-exchange spectroscopy spin-lock pulse train [Hwang, T.-L., Mori, S., Shaka, A. J. & van Zijl, P. C. M. (1997) J. Am. Chem. Soc. 119, 6203-6204] to test for psiNH1-water cross-relaxation effects within two RNA helices: (i) a complementary duplex, in which psi is not associated with structural change, and (ii) an RNA duplex representing the eukaryotic pre-mRNA branch-site helix from Saccharomyces cerevisiae, in which a conserved psi extrudes the branch-site adenosine from the helix. Our data implicate a water-psiNH1 hydrogen bond both in stabilizing the complementary helix and in favoring formation of the unique structure of the branch-site helix. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 20 |
| Volume Number | 99 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2002-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Protons Pseudouridine Chemistry Adenosine Metabolism Crystallography, X-Ray Hydrogen Bonding Magnetic Resonance Spectroscopy Models, Chemical Models, Genetic Models, Molecular Molecular Sequence Data RNA RNA Processing, Post-Transcriptional RNA, Complementary RNA, Messenger Saccharomyces Cerevisiae Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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