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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Calderon-amador, Juana Flores-langarica, Adriana Meza-perez, Selene Estrada-garcia, Teresa Macpherson, Gordon Flores-romo, Leopoldo Lebecque, Serge Steinman, Ralph M. Saeland, Sem |
| Description | Author Affiliation: Flores-Langarica A ( Department of Immunology, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala, Casco de Santo Tomás, 11340, Mexico.); |
| Abstract | Dendritic cells (DCs) are located at body surfaces such as the skin, respiratory and genital tracts, and intestine. To further analyze intestinal DCs, we adapted an epidermal sheet separation technique and obtained two intestinal layers, facing the lumen and serosa. Unexpectedly, immunolabeling of the layer toward the serosa revealed a regular, dense, planar network of cells with prominent dendritic morphology within the external muscular layer and with increasing frequency along the length of the intestine. Direct examination of the serosal-disposed layers showed a significant fraction of the DCs to express DEC-205/CD205, CD11c, Langerin/CD207, Fcgamma receptor/CD16/32, CD14, and low levels of activation markers, CD25, CD80, CD86, and CD95. By more sensitive FACS analyses, cells from this layer contained two CD11c(+) populations of CD45(+) CD205(+), CD19(-) leukocytes, MHC II(+) and MHC II(-). When ovalbumin conjugated to an anti-DEC-205 antibody was injected into mice, the conjugate targeted to these DCs, which upon isolation were able to stimulate ovalbumin-specific, CD4(+) and CD8(+) T cell antigen receptor-transgenic T cells. In vivo, these DCs responded to two microbial stimuli, systemic LPS and oral live bacteria, by up-regulating CD80, CD86, DEC-205, and Langerin within 12 h. This network of DCs thus represents a previously unrecognized antigen-presenting cell system in the intestine. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 52 |
| Volume Number | 102 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2005-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Dendritic Cells Cytology Intestines Muscles Animals Antigen Presentation Antigen-Presenting Cells Antigens, CD Chemistry Antigens, CD11c Biosynthesis Antigens, CD80 Antigens, CD86 Cell Proliferation Cell Separation Metabolism Edetic Acid Flow Cytometry Immunohistochemistry Anatomy & Histology Lectins, C-Type Leukocytes Lipopolysaccharides Lymph Nodes Pathology Mice Mice, Inbred BALB C Mice, Inbred C57BL Minor Histocompatibility Antigens Phenotype Receptors, Cell Surface Time Factors Up-Regulation Research Support, N.I.H., Extramural Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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