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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lavitrano, Marialuisa Manzini, Stefano Vargiolu, Alessia Cerrito, Maria Grazia Giovannoni, Roberto Donini, Pierluigi Lipps, Hans J. Zannoni, Augusta Forni, Monica Bacci, Maria Laura Stehle, Isa M. Papa, Michele Bianco, Maria Rosaria |
| Description | Author Affiliation: Manzini S ( Department of Surgical Sciences, University of Milano-Bicocca, 20052 Milan,Italy.); |
| Abstract | Genetic modification of cells and animals is an invaluable tool for biotechnology and biomedicine. Currently, integrating vectors are used for this purpose. These vectors, however, may lead to insertional mutagenesis and variable transgene expression and can undergo silencing. Scaffold/matrix attachment region-based vectors are nonviral expression systems that replicate autonomously in mammalian cells, thereby making possible safe and reliable genetic modification of higher eukaryotic cells and organisms. In this study, genetically modified pig fetuses were produced with the scaffold/matrix attachment region-based vector pEPI, delivered to embryos by the sperm-mediated gene transfer method. The pEPI vector was detected in 12 of 18 fetuses in the different tissues analyzed and was shown to be retained as an episome. The reporter gene encoded by the pEPI vector was expressed in 9 of 12 genetically modified fetuses. In positive animals, all tissues analyzed expressed the reporter gene; moreover in these tissues, the positive cells were on the average 79%. The high percentage of EGFP-expressing cells and the absence of mosaicism have important implications for biotechnological and biomedical applications. These results are an important step forward in animal transgenesis and can provide the basis for the future development of germ-line gene therapy. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 47 |
| Volume Number | 103 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2006-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Animals, Genetically Modified Genetic Vectors Genetics Plasmids Animals Fetus Anatomy & Histology Physiology Gene Transfer Techniques Metabolism Green Fluorescent Proteins Spermatozoa Swine Tissue Distribution Transgenes Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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