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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pohlschröder, Mechthild Naldrett, Mike Widdick, David A. Chandra, Govind Bottrill, Andrew Dilks, Kieran Palmer, Tracy |
| Description | Author Affiliation: Widdick DA ( Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, United Kingdom.); |
| Abstract | The twin-arginine translocation (Tat) pathway is a protein transport system for the export of folded proteins. Substrate proteins are targeted to the Tat translocase by N-terminal signal peptides harboring a distinctive R-R-x-Phi-Phi 'twin-arginine' amino acid motif. Using a combination of proteomic techniques, the protein contents from the cell wall of the model Gram-positive bacterium Streptomyces coelicolor were identified and compared with that of mutant strains defective in Tat transport. The proteomic experiments pointed to 43 potentially Tat-dependent extracellular proteins. Of these, 25 were verified as bearing bona fide Tat-targeting signal peptides after independent screening with a facile, rapid, and sensitive reporter assay. The identified Tat substrates, among others, include polymer-degrading enzymes, phosphatases, and binding proteins as well as enzymes involved in secondary metabolism. Moreover, in addition to predicted extracellular substrates, putative lipoproteins were shown to be Tat-dependent. This work provides strong experimental evidence that the Tat system is used as a major general export pathway in Streptomyces. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 47 |
| Volume Number | 103 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2006-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Arginine Metabolism Bacterial Proteins Membrane Transport Proteins Protein Transport Physiology Streptomyces Coelicolor Genetics Escherichia Coli Proteins Glycoside Hydrolases Molecular Sequence Data Mutation Phenotype Protein Sorting Signals Proteomics Cytology Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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