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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nesvizhskii, Alexey I. Aebersold, Ruedi Rani, P. Geetha Hahn, Steven Kim, Bong Ranish, Jeffrey A. |
| Description | Author Affiliation: Kim B ( Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA.); |
| Abstract | In this article, we provide direct evidence that the evolutionarily conserved transcription elongation factor TFIIS functions during preinitiation complex assembly. First, we identified TFIIS in a mass spectrometric screen of RNA polymerase II (Pol II) preinitiation complexes (PICs). Second, we show that the association of TFIIS with a promoter depends on functional PIC components including Mediator and the SAGA complex. Third, we demonstrate that TFIIS is required for efficient formation of active PICs. Using truncation mutants of TFIIS, we find that the Pol II-binding domain is the minimal domain necessary to stimulate PIC assembly. However, efficient formation of active PICs requires both the Pol II-binding domain and the poorly understood N-terminal domain. Importantly, Domain III, which is required for the elongation function of TFIIS, is dispensable during PIC assembly. The results demonstrate that TFIIS is a PIC component that is required for efficient formation and/or stability of the complex. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 41 |
| Volume Number | 104 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2007-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | RNA Polymerase II Genetics Metabolism Saccharomyces Cerevisiae Proteins Saccharomyces Cerevisiae Transcriptional Elongation Factors Binding Sites DNA, Fungal Multiprotein Complexes Promoter Regions, Genetic Protein Structure, Tertiary Chemistry Sequence Deletion Tandem Mass Spectrometry Transcription, Genetic Research Support, N.I.H., Extramural Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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