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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kaznessis, Yiannis N. Dunny, Gary M. Johnson, Christopher M. Shu, Che-chi Ramkrishna, Doraiswami Chatterjee, Anushree Hu, Wei-shou |
| Description | Author Affiliation: Chatterjee A ( Department of Chemical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.); |
| Abstract | Convergent gene pairs with head-to-head configurations are widespread in both eukaryotic and prokaryotic genomes and are speculated to be involved in gene regulation. Here we present a unique mechanism of gene regulation due to convergent transcription from the antagonistic prgX/prgQ operon in Enterococcus faecalis controlling conjugative transfer of the antibiotic resistance plasmid pCF10 from donor cells to recipient cells. Using mathematical modeling and experimentation, we demonstrate that convergent transcription in the prgX/prgQ operon endows the system with the properties of a robust genetic switch through premature termination of elongating transcripts due to collisions between RNA polymerases (RNAPs) transcribing from opposite directions and antisense regulation between complementary counter-transcripts. Evidence is provided for the presence of truncated RNAs resulting from convergent transcription from both the promoters that are capable of sense-antisense interactions. A mathematical model predicts that both RNAP collision and antisense regulation are essential for a robust bistable switch behavior in the control of conjugation initiation by prgX/prgQ operons. Moreover, given that convergent transcription is conserved across species, the mechanism of coupling RNAP collision and antisense interaction is likely to have a significant regulatory role in gene expression. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 23 |
| Volume Number | 108 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2011-06-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Conjugation, Genetic Genetics Enterococcus Faecalis Plasmids Transcription, Genetic Algorithms Bacterial Proteins Blotting, Northern DNA-Directed RNA Polymerases Gene Expression Regulation, Bacterial Models, Genetic Molecular Sequence Data Operon Protein Sorting Signals Repressor Proteins Research Support, N.I.H., Extramural Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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