| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Can, Ali Filkins, Robert J. Larsen, Melinda Bordwell, Alexander Corwin, Alex Zhang, Jingyu Gerdes, Michael J. Li, Qing Rittscher, Jens Sarachan, Brion D. Sevinsky, Christopher J. Hollman, Denise Pang, Zhengyu Mcdonough, Elizabeth Ginty, Fiona Kaanumalle, Sireesha Seppo, Antti Adak, Sudeshna Santamaria-pang, Alberto Mcculloch, Colin C. Seel, Maximilian L. Montalto, Michael C. Shaikh, Kashan Kenny, Kevin Sood, Anup Lowes, Christina Bello, Musodiq O. Dinn, Sean Kamath, Vidya Lazare, Michael Sui, Yunxia |
| Description |
Author Affiliation: Gerdes MJ ( Cell Biology Laboratory, GE Global Research Center, Niskayuna, NY 12309, USA.); |
| Abstract |
Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances in understanding the biological basis of health and disease. Here we present a multiplexed fluorescence microscopy method (MxIF) for quantitative, single-cell, and subcellular characterization of multiple analytes in formalin-fixed paraffin-embedded tissue. Chemical inactivation of fluorescent dyes after each image acquisition round allows reuse of common dyes in iterative staining and imaging cycles. The mild inactivation chemistry is compatible with total and phosphoprotein detection, as well as DNA FISH. Accurate computational registration of sequential images is achieved by aligning nuclear counterstain-derived fiducial points. Individual cells, plasma membrane, cytoplasm, nucleus, tumor, and stromal regions are segmented to achieve cellular and subcellular quantification of multiplexed targets. In a comparison of pathologist scoring of diaminobenzidine staining of serial sections and automated MxIF scoring of a single section, human epidermal growth factor receptor 2, estrogen receptor, p53, and androgen receptor staining by diaminobenzidine and MxIF methods yielded similar results. Single-cell staining patterns of 61 protein antigens by MxIF in 747 colorectal cancer subjects reveals extensive tumor heterogeneity, and cluster analysis of divergent signaling through ERK1/2, S6 kinase 1, and 4E binding protein 1 provides insights into the spatial organization of mechanistic target of rapamycin and MAPK signal transduction. Our results suggest MxIF should be broadly applicable to problems in the fields of basic biological research, drug discovery and development, and clinical diagnostics. |
| ISSN |
00278424 |
| e-ISSN |
10916490 |
| Journal |
Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number |
29 |
| Volume Number |
110 |
| Language |
English |
| Publisher |
National Academy of Sciences |
| Publisher Date |
2013-07-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Tumor Markers, Biological Metabolism Breast Neoplasms Diagnosis Colonic Neoplasms Formaldehyde Microscopy, Fluorescence Paraffin Embedding 3,3'-Diaminobenzidine Cell Line, Tumor Image Processing, Computer-Assisted Immunohistochemistry In Situ Hybridization, Fluorescence Receptor, ErbB-2 Receptors, Androgen Receptors, Estrogen Statistics, Nonparametric Tumor Suppressor Protein P53 Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Multidisciplinary |
