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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rosenwaks, Zev Rafii, Shahin Liu, Ying Geng, Fuqiang Xiang, Jenny Elemento, Olivier Allis, C. David Wen, Duancheng Banaszynski, Laura A. Noh, Kyung-min |
| Description | Author Affiliation: Wen D ( Department of Genetic Medicine, Ansary Stem Cell Institute,Howard Hughes Medical Institute,Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine.); Banaszynski LA ( Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065.); Liu Y ( Department of Genetic Medicine, Ansary Stem Cell Institute,Howard Hughes Medical Institute.); Geng F ( Department of Genetic Medicine, Ansary Stem Cell Institute,Howard Hughes Medical Institute.); Noh KM ( Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065.); Xiang J ( Genomics Resources Core Facility, and.); Elemento O ( Department of Physiology, Weill Cornell Medical College, New York, NY 10065); Rosenwaks Z ( Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine.); Allis CD ( Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065 alliscd@rockefeller.edu srafii@med.cornell.edu.); Rafii S ( Department of Genetic Medicine, Ansary Stem Cell Institute,Howard Hughes Medical Institute, alliscd@rockefeller.edu srafii@med.cornell.edu.); |
| Abstract | Mature oocyte cytoplasm can reprogram somatic cell nuclei to the pluripotent state through a series of sequential events including protein exchange between the donor nucleus and ooplasm, chromatin remodeling, and pluripotency gene reactivation. Maternal factors that are responsible for this reprogramming process remain largely unidentified. Here, we demonstrate that knockdown of histone variant H3.3 in mouse oocytes results in compromised reprogramming and down-regulation of key pluripotency genes; and this compromised reprogramming for developmental potentials and transcription of pluripotency genes can be rescued by injecting exogenous H3.3 mRNA, but not H3.2 mRNA, into oocytes in somatic cell nuclear transfer embryos. We show that maternal H3.3, and not H3.3 in the donor nucleus, is essential for successful reprogramming of somatic cell nucleus into the pluripotent state. Furthermore, H3.3 is involved in this reprogramming process by remodeling the donor nuclear chromatin through replacement of donor nucleus-derived H3 with de novo synthesized maternal H3.3 protein. Our study shows that H3.3 is a crucial maternal factor for oocyte reprogramming and provides a practical model to directly dissect the oocyte for its reprogramming capacity. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 20 |
| Volume Number | 111 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2014-05-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Nucleus Metabolism Cellular Reprogramming Gene Expression Regulation, Developmental Histones Chemistry Oocytes Cytology Animals Chromatin Cytoplasm Mice Nuclear Transfer Techniques RNA, Small Interfering Sequence Analysis, RNA Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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