NDLI: Whole transcriptome analysis: what are we still missing?

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Whole transcriptome analysis: what are we still missing?

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Saxena, Alka Carninci, Piero
Description	Country affiliation: Japan Author Affiliation: Saxena A ( Omics Science Center, RIKEN Yokohama Institute, Tsurumi, Japan.)
Abstract	New technologies such as tag-based sequencing and tiling arrays have provided unique insights into the transcriptional output of cells. Many new RNA classes have been uncovered in the past decade, despite limitations in current technologies. Even as the repertoire of known functional elements of the transcriptome increases and contemporary technologies become mainstream, inadequacies in conventional protocols for library preparation, sequencing and mapping continue to hamper revelation of the entire transcriptome of cells. In this article, we review current protocols and outline their deficiencies. We also provide our view on what we may be overlooking in the transcriptome, despite exhaustive investigations, and indicate future areas of technological development and research.
File Format	HTM / HTML
ISSN	19395094
Issue Number	5
Volume Number	3
e-ISSN	1939005X
Journal	Wiley Interdisciplinary Reviews: Systems Biology and Medicine
Language	English
Publisher	Wiley
Publisher Date	2011-09-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Computational Biology Transcriptome Dna Primers Metabolism Dna-directed Dna Polymerase Rna, Untranslated Classification Genetics Rna-directed Dna Polymerase Sequence Analysis Methods Journal Article Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Review
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry, Genetics and Molecular Biology

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