NDLI: Differentiation induction of cardiac c-kit positive cells from rat heart into sinus node-like cells by 5-azacytidine.

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Differentiation induction of cardiac c-kit positive cells from rat heart into sinus node-like cells by 5-azacytidine.

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Differentiation induction of cardiac c-kit positive cells from rat heart into sinus node-like cells by 5-azacytidine.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, Jun Huang, Congxin Wu, Pan Yang, Jing Song, Tao Chen, Yongjun Fan, Xinrong Wang, Ten
Description	Author Affiliation: Zhang J ( Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, PR China.)
Abstract	Cardiac stem cells (CSCs) can differentiate into cardiac phenotypes representing early cardiomyocyte-like cells. However, CSCs-derived specification into sinus nodal cells is rarely known. Using the demethylating agent, 5-Aazacytidine (5-Aza), we tried to follow the process of cardiac specialization into sinus node-like cells. The c-kit+ cells were isolated from 1-month-old rat hearts and sorted by a flow cytometry cell sorting system. Then, analyses of immunofluorescence and reverse transcription polymerase chain reaction were performed on the cells. The sorted c-kit+ cell are self-renewing and clonogenic. Some of the c-kit+ cell could spontaneously express GATA-4, cardiac troponin I (cTnI), smooth muscle actin (SMA) and CD31. At week 8, the cells treated with 10 µM 5-Aza expressed GATA-4 and cTnI at rates of 31.4±2.6% and 32.6±8.5%. Correspondingly, the rates were 21.4±8.1% and 18.7±4.3% in the cells without 5-Aza. The difference of GATA-4 or cTnI expression rate between two groups was of significance respectively, P<0.05. The cultured cells could express mRNA of GATA4, hyperpolarization-activated cyclic nucleotide-gated 2 (HCN2) and HCN4. The mRNA expression was also up-regulated in cells treated with 10 µM 5-Aza and growth factors. By week 2 after cell sorting, inward currents could be recorded in a fraction of the cultured cells. In conclusion, 10 µM 5-Aza could promote the differentiation of c-kit+ cells into sinus node-like cells. 5-Aza-mediated differentiation seems to be helpful in future cell therapy for the patients suffering from loss of sinus node cells.
File Format	HTM / HTML
ISSN	00408166
Issue Number	2
Volume Number	43
e-ISSN	15323072
Journal	Tissue and Cell
Language	English
Publisher	Elsevier
Publisher Date	2011-04-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Cell Biology Azacitidine Pharmacology Cell Differentiation Drug Effects Enzyme Inhibitors Heart Proto-oncogene Proteins C-kit Metabolism Sinoatrial Node Animals Biological Markers Cells, Cultured Gata4 Transcription Factor Genetics Hyperpolarization-activated Cyclic Nucleotide-gated Channels Immunohistochemistry Ion Channels Methylation Potassium Channels Rats Journal Article
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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