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A hyperpolarization-activated, cyclic nucleotide-gated, (Ih-like) cationic current and HCN gene expression in renal inner medullary collecting duct cells.

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A hyperpolarization-activated, cyclic nucleotide-gated, (Ih-like) cationic current and HCN gene expression in renal inner medullary collecting duct cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bolívar, Juan J. Tapia, Dagoberto Arenas, Gabina Castañón-Arreola, Mauricio Torres, Haydee Galarraga, Elvira
Description	Author Affiliation: Bolívar JJ ( Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City DF, 04510, México. jjboliv@servidor.unam.mx)
Abstract	The cation conductancein primary cultures of rat renal inner medullary collecting duct was studied using perforated-patch and conventional whole cell clamp techniques. Hyperpolarizations beyond -60 mV induced a time-dependent inward nonselective cationic current (I(vti)) that resembles the well-known hyperpolarization-activated, cyclic nucleotide-gated I(h) and I(f) currents. I(vti) showed a half-maximal activation around -102 mV with a slope factor of 25 mV. It had a higher conductance (but, at its reversal potential, not a higher permeability) for K(+) than for Na(+) (gK(+)/gNa(+) = 1.5), was modulated by cAMP and blocked by external Cd(2+) (but not Cs(+) or ZD-7288), and potentiated by a high extracellular K(+) concentration. We explored the expression of the I(h) channel genes (HCN1 to -4) by RT-PCR. The presence of transcripts corresponding to the HCN1, -2, and -4 genes was observed in both the cultured cells and kidney inner medulla. Western blot analysis with HCN2 antibody showed labeling of approximately 90- and approximately 120-kDa proteins in samples from inner medulla and cultured cells. Immunocytochemical analysis of cell cultures and inner medulla showed the presence of HCN immunoreactivity partially colocalized with the Na(+)-K(+)-ATPase at the basolateral membrane of collecting duct cells. This is the first evidence of an I(h)-like cationic current and HCN immunoreactivity in either kidney or any other nonexcitable mammalian cells.
File Format	HTM / HTML
ISSN	03636143
e-ISSN	15221563
Journal	American Journal of Physiology - Cell Physiology
Issue Number	4
Volume Number	294
Language	English
Publisher	American Physiological Society
Publisher Date	2008-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Cell Biology Cyclic Nucleotide-gated Cation Channels Metabolism Gene Expression Regulation Physiology Kidney Medulla Cytology Kidney Tubules, Collecting Potassium Channels Animals Cells, Cultured Cyclic Amp Genetics Hyperpolarization-activated Cyclic Nucleotide-gated Channels Ion Channel Gating Ultrastructure Membrane Potentials Potassium Pharmacology Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology

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A hyperpolarization-activated, cyclic nucleotide-gated, (Ih-like) cationic current and HCN gene expression in renal inner medullary collecting duct cells.

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