NDLI: Insights into possible cell-death markers in the diatom Skeletonema marinoi in response to senescence and silica starvation.

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Insights into possible cell-death markers in the diatom Skeletonema marinoi in response to senescence and silica starvation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Orefice, Ida Lauritano, Chiara Procaccini, Gabriele Ianora, Adrianna Romano, Giovanna
Description	Author Affiliation: Orefice I ( Stazione Zoologica Anton Dohrn, Villa Comunale 1, 80121 Napoli, Italy. Electronic address: ida.orefice@szn.it.); Lauritano C ( Stazione Zoologica Anton Dohrn, Villa Comunale 1, 80121 Napoli, Italy. Electronic address: chiara.lauritano@szn.it.); Procaccini G ( Stazione Zoologica Anton Dohrn, Villa Comunale 1, 80121 Napoli, Italy. Electronic address: gabriele.procaccini@szn.it.); Ianora A ( Stazione Zoologica Anton Dohrn, Villa Comunale 1, 80121 Napoli, Italy. Electronic address: adrianna.ianora@szn.it.); Romano G ( Stazione Zoologica Anton Dohrn, Villa Comunale 1, 80121 Napoli, Italy. Electronic address: giovanna.romano@szn.it.)
Abstract	Diatoms are a hugely diverse microalgal class, which possesses unique biological features and complex metabolic pathways and may activate sophisticated mechanisms to respond to environmental changes. Abiotic stress factors may limit growth rate of diatoms, but may also trigger intracellular signaling pathways that cause cells to undergo programmed cell death (PCD). Here we investigate the gene expression of different target genes related to cell death, namely programmed cell death 4 (PDCD4), tumor susceptibility gene 101 (TSG101), developmental and cell death (DCD) domain, death specific protein (DSP) and metacaspase (MC), using RT-qPCR in the cosmopolitan coastal centric diatom species Skeletonema marinoi, which contributes significantly to phytoplankton blooms in temperate waters. To this end, we undertook a detailed study of the best reference genes to analyze gene expression in S. marinoi under different experimental conditions (i.e. in different growth phases or under silica starvation). Results showed that DSP gene expression had a clear and constant increase along the S. marinoi growth curve reaching its maximum during the senescent phase. On the contrary, PDCD4, DCD, TSG101 and MC did not show any significant variation. These findings indicate that the DSP gene is a possible PCD marker induced by aging in this diatom species. In contrast, levels of DSP transcripts induced by silica starvation were relatively low compared to those induced by cell aging suggesting differential activation and/or regulation of the PCD machinery in response to different stressful conditions. Our study also expands the list of reference genes available for the diatom S. marinoi for normalization of RT-qPCR data of cells cultivated under different growth phases or under silica starvation.
File Format	HTM / HTML
ISSN	18747787
Volume Number	24 Pt 1
e-ISSN	18767478
Journal	Marine Genomics
Language	English
Publisher	Elsevier
Publisher Date	2015-12-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Genetics Discipline Biology Cell Aging Physiology Cell Death Diatoms Metabolism Silicon Dioxide Biological Markers Cytology Gene Expression Regulation Drug Effects Chemistry Transcriptome Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Aquatic Science

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Si–C interactions during degradation of the diatom Skeletonema marinoi

Antioxidant and Photoprotection Networking in the Coastal Diatom Skeletonema marinoi.

Insights into possible cell-death markers in the diatom Skeletonema marinoi in response to senescence and silica starvation.

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Insights into possible cell-death markers in the diatom Skeletonema marinoi in response to senescence and silica starvation.