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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Wei Cao, Jing-tao Liu, Hai-yu |
| Description | Country affiliation: China Author Affiliation: Zhang W ( Department of Respiratory Diseases, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province 250011, China. 05320835zh@sina.com) |
| Abstract | OBJECTIVE: To explore the effects of wind and dampness pathogens on cytokines in the lung tissue of rats with cold syndrome due to different gradient cold pathogens. METHODS: One hundred and four Wistar rats of SPF grade were randomly divided into 13 groups: normal temperature group, six cold pathogen groups and six cold plus wind-dampness pathogen (wind of grade 5 and 90%-100% relative humidity) groups. The cold pathogens were constant low temperature (including 10 degrees C, 0 degree C, -10 degrees C) and temperature change (including 20 to 10 degrees C, 20 to 0 degrees C, and 20 to -10 degrees C). The rats in different groups were kept in a temperature-controlled box under the corresponding condition for 2 hours on the first day of experiment. Then, the rats were all raised in normal temperature for 4 days and the rats' behaviors were observed. The contents of tumor necrosis factor alpha (TNF-alpha), interleukin-6(IL-6) and interleukin-4 (IL-4) in lung homogenate were measured by radioimmunoassay and the content of interferon-gamma (IFN-gamma) was detected by enzyme-linked immunosorbent assay. RESULTS: In comparison with cold pathogen groups, contents of TNF-alpha, IL-6 and IL-4 were obviously increased in lung homogenate of rats in cold plus wind-dampness pathogen groups (P<0.01), and the content of IFN-gamma and IFN-gamma/IL-4 ratio were obviously decreased (P<0.01). CONCLUSION: Wind-dampness pathogen can seriously aggravate the injury to lung tissue caused by cold pathogen, and the unbalance of Th(1)/Th(2) in lung homogenate of rats. |
| File Format | HTM / HTML |
| ISSN | 20954964 |
| Journal | Journal of Chinese Integrative Medicine |
| Issue Number | 7 |
| Volume Number | 6 |
| Language | Chinese |
| Publisher | Science Press |
| Publisher Date | 2008-07-01 |
| Publisher Place | China |
| Access Restriction | Open |
| Subject Keyword | Discipline Therapeutics Cold Temperature Cytokines Metabolism Diagnosis, Differential Lung Medicine, Chinese Traditional Animals Humidity Interferon-gamma Interleukin-4 Interleukin-6 Random Allocation Rats, Wistar T-lymphocytes, Helper-inducer Immunology Tumor Necrosis Factor-alpha Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Complementary and Alternative Medicine |
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