NDLI: A Highly Sensitive Oligonucleotide Hybridization Assay for Klebsiella pneumoniae Carbapenemase with the Probes on a Gold Nanoparticles Modified Glassy Carbon Electrode.

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A Highly Sensitive Oligonucleotide Hybridization Assay for Klebsiella pneumoniae Carbapenemase with the Probes on a Gold Nanoparticles Modified Glassy Carbon Electrode.

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A Highly Sensitive Oligonucleotide Hybridization Assay for Klebsiella pneumoniae Carbapenemase with the Probes on a Gold Nanoparticles Modified Glassy Carbon Electrode.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pan, Hong-zhi Yu, Hong- Wei Wang, Na Zhang, Ze Wan, Guang-Cai Liu, Hao Guan, Xue Chang, Dong
Description	Author Affiliation: Pan HZ ( Public Health School, Harbin Medical University, No. 157 Baojian Rd, Nangang District, Harbin, Heilongjiang Province, 150081, People's Republic of China.)
Abstract	To develop a new electrochemical DNA biosensor for determination of Klebsiella pneumoniae carbapenemase, a highly sensitive and selective electrochemical biosensor for DNA detection was constructed based on a glassy carbon electrode (GCE) modified with gold nanoparticles (Au-nano). The Au-nano/GCE was characterized by scanning electromicroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The hybridization detection was measured by differential pulse voltammetry using methylene blue as the hybridization indicator. The dynamic range of detection of the sensor for the target DNA sequences was from 1 × 10(-11) to 1 × 10(-8) M, with an LOD of 1 × 10(-12) M. The DNA biosensor had excellent specificity for distinguishing complementary DNA sequence in the presence of non-complementary and mismatched DNA sequence. The Au-nano/GCE showed significant improvement in electrochemical characteristics, and this biosensor was successfully applied for determination of K. pneumoniae.
File Format	HTM / HTML
ISSN	10603271
Issue Number	6
Volume Number	98
e-ISSN	19447922
Journal	Journal of AOAC International
Language	English
Publisher	AOAC International
Publisher Date	2015-11-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Analytical Discipline Chemistry Techniques Bacterial Proteins Analysis Biosensing Techniques Methods Klebsiella Pneumoniae Enzymology Metal Nanoparticles Chemistry Oligonucleotides Genetics Beta-lactamases Carbon Electrochemistry Electrodes Gold Nucleic Acid Hybridization Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Analytical Chemistry Pharmacology Food Science Agronomy and Crop Science

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