NDLI: Automated Aflatoxin Analysis Using Inline Reusable Immunoaffinity Column Cleanup and LC-Fluorescence Detection.

Please wait, while we are loading the content...

Year: 2017 Volume: 100

Year: 2016 Volume: 99

Year: 2015 Volume: 98

Year: 2015 Volume: 98 Issue: 6

Determination of Nine Fungicides in Grape and Wine Using QuEChERS Extraction and LC/MS/MS Analysis.

Evaluation of the Determination of Free Urea in Water-Soluble Liquid Fertilizers Containing Urea and Ureaforms by Urease and HPLC Methods.

Determination of Isoflavone Content in SRM 3238 Using Liquid Chromatography-Particle Beam/Electron Ionization Mass Spectrometry.

Evaluation of Different Light Conditions in the Working Environment for Handling Photosensitive and Thermolabile Compounds.

Validated HPLC and Ultra-HPLC Methods for Determination of Dronedarone and Amiodarone Application for Counterfeit Drug Analysis.

Development and Validation of a Reliable and Robust Method for the Analysis of Cannabinoids and Terpenes in Cannabis.

Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography.

Rapid Enantiomeric Separation and Quantitation of Levetiracetam on -Acid Glycoprotein (AGP) Chiral Stationary Phase by High-Performance Liquid Chromatography.

Rapid Electroanalytical Method for Determination of Nebivolol at a Boron-Doped Diamond Electrode.

Simultaneous Determination of Eight Hypotensive Drugs of Various Chemical Groups in Pharmaceutical Preparations by HPLC-DAD.

Crystal Diagnostics Xpress™ E7 STEC Kit for the Rapid Multiplex Detection of E. coli O157 and non-O157 Shiga toxin-producing E. coli.

Determination of ß-N-methylamino-L-alanine, N-(2-aminoethyl)glycine, and 2,4-diaminobutyric acid in Food Products Containing Cyanobacteria by Ultra-Performance Liquid Chromatography and Tandem Mass Spectrometry: Single-Laboratory Validation.

Determination of Ochratoxin A in Pig Kidneys by Immunoaffinity Cleanup and Ultra-High Performance Liquid Chromatography.

QuickTox™ Kit for QuickScan Aflatoxin FREE.

Automated Aflatoxin Analysis Using Inline Reusable Immunoaffinity Column Cleanup and LC-Fluorescence Detection.

A Rapid Method for Determination of the Main Conjugated Linoleic Acid Precursors (C18:2 n-6 and C18:3 n-3) in Forage by Capillary Zone Electrophoresis with Ultraviolet Detection Using Gas Chromatography with Flame Ionization Detection as a Comparative Method.

Lipid Characterization of White, Dark, and Milk Chocolates by FT-Raman Spectroscopy and Capillary Zone Electrophoresis.

GlutenTox® Pro Test for the Detection of Gluten in Select Foods and Surfaces.

Rapid Quantification of Four Anthocyanins in Red Grape Wine by Hydrophilic Interaction Liquid Chromatography/Triple Quadrupole Linear Ion Trap Mass Spectrometry.

A Rapid Method for Analysis of Phenylalanine in Cereal Products by MEKC-UV Using LC/MS/MS as a Comparative Method.

A Real-Time PCR Method Targeting Camel Ingredient for Food Authentication.

Detection of Adulterated Vegetable Oils Containing Waste Cooking Oils Based on the Contents and Ratios of Cholesterol, ß-Sitosterol, and Campesterol by Gas Chromatography/Mass Spectrometry.

Determination of Vitamin B12 in Infant, Adult, and Pediatric Formulas by HPLC-UV and Column Switching: Collaborative Study, Final Action 2011.10.

Determination of Myo-Inositol in Infant, Pediatric, and Adult Formulas by Liquid Chromatography-Pulsed Amperometric Detection with Column Switching: Collaborative Study, Final Action 2011.18.

Determination of Labeled Fatty Acids Content in Milk Products, Infant Formula, and Adult/Pediatric Nutritional Formula by Capillary Gas Chromatography: Single-Laboratory Validation, First Action 2012.13.

Pantothenic Acid (Vitamin B5) in Infant Formula and Adult/ Pediatric Nutritional Formula by Ultra-High Pressure Liquid Chromatography/Tandem Mass Spectrometry Method: Collaborative Study, Final Action 2012.16.

Determination of Chromium, Selenium, and Molybdenum in Infant Formula and Adult Nutritional Products by Inductively Coupled Plasma/Mass Spectrometry: Collaborative Study, Final Action 2011.19.

Determination of Minerals and Trace Elements in Infant Formula and Adult/Pediatric Nutritional Formula by Inductively Coupled Plasma/Mass Spectrometry A Performance Evaluation: Single-Laboratory Validation, First Action 2015.06.

Data Quality in Biofilm High-Throughput Routine Analysis: Intralaboratory Protocol Adaptation and Experiment Reproducibility.

A Highly Sensitive Oligonucleotide Hybridization Assay for Klebsiella pneumoniae Carbapenemase with the Probes on a Gold Nanoparticles Modified Glassy Carbon Electrode.

Separation and Enrichment of Gold in Water, Geological and Environmental Samples by Solid Phase Extraction on Multiwalled Carbon Nanotubes Prior to its Determination by Flame Atomic Absorption Spectrometry.

Determination of Dufulin Residue in Vegetables, Rice, and Tobacco Using Liquid Chromatography with Tandem Mass Spectrometry.

Ultra-Performance Liquid Chromatographic Determination of Manufacturing Intermediates and Subsidiary Colors in D&C Red No. 6, D&C Red No. 7, and Their Lakes.

An Interlaboratory Comparative Study on the Quantitative Determination of Glyphosate at Low Levels in Wheat Flour.

Systematic Robustness Testing of a Liquid Chromatographic Method: A Case Study.

Year: 2015 Volume: 98 Issue: 5

Year: 2015 Volume: 98 Issue: 4

Year: 2015 Volume: 98 Issue: 3

Year: 2015 Volume: 98 Issue: 2

Year: 2015 Volume: 98 Issue: 1

Year: 2014 Volume: 97

Year: 2012 Volume: 95

Year: 2011 Volume: 94

Year: 2010 Volume: 93

Year: 2009 Volume: 92

Year: 2008 Volume: 91

Year: 2007 Volume: 90

Year: 2006 Volume: 89

Year: 2005 Volume: 88

Year: 2004 Volume: 87

Year: 2003 Volume: 86

Automated Aflatoxin Analysis Using Inline Reusable Immunoaffinity Column Cleanup and LC-Fluorescence Detection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rhemrev, Ria Pazdanska, Monika Marley, Elaine Biselli, Scarlett Staiger, Simone
Description	Country affiliation: United kingdom Author Affiliation: Rhemrev R ( R-Biopharm Rhone Ltd, Block 10, Todd Campus, West of Scotland Science Park, Acre Rd, Glasgow G20 OXA, UK.)
Abstract	A novel reusable immunoaffinity cartridge containing monoclonal antibodies to aflatoxins coupled to a pressure resistant polymer has been developed. The cartridge is used in conjunction with a handling system inline to LC with fluorescence detection to provide fully automated aflatoxin analysis for routine monitoring of a variety of food matrixes. The handling system selects an immunoaffinity cartridge from a tray and automatically applies the sample extract. The cartridge is washed, then aflatoxins B1, B2, G1, and G2 are eluted and transferred inline to the LC system for quantitative analysis using fluorescence detection with postcolumn derivatization using a KOBRA® cell. Each immunoaffinity cartridge can be used up to 15 times without loss in performance, offering increased sample throughput and reduced costs compared to conventional manual sample preparation and cleanup. The system was validated in two independent laboratories using samples of peanuts and maize spiked at 2, 8, and 40 µg/kg total aflatoxins, and paprika, nutmeg, and dried figs spiked at 5, 20, and 100 µg/kg total aflatoxins. Recoveries exceeded 80% for both aflatoxin B1 and total aflatoxins. The between-day repeatability ranged from 2.1 to 9.6% for aflatoxin B1 for the six levels and five matrixes. Satisfactory Z-scores were obtained with this automated system when used for participation in proficiency testing (FAPAS®) for samples of chilli powder and hazelnut paste containing aflatoxins.
File Format	HTM / HTML
ISSN	10603271
Issue Number	6
Journal	Journal of AOAC International
Volume Number	98
e-ISSN	19447922
Language	English
Publisher	AOAC International
Publisher Date	2015-11-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Analytical Discipline Chemistry Techniques Aflatoxins Analysis Chromatography, Affinity Methods Chromatography, Liquid Fluorescence Limit Of Detection Journal Article
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Analytical Chemistry Pharmacology Food Science Agronomy and Crop Science

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in