NDLI: Wheat germ agglutinin staining as a suitable method for detection and quantification of fibrosis in cardiac tissue after myocardial infarction.

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Wheat germ agglutinin staining as a suitable method for detection and quantification of fibrosis in cardiac tissue after myocardial infarction.

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Wheat germ agglutinin staining as a suitable method for detection and quantification of fibrosis in cardiac tissue after myocardial infarction.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Emde, B. Heinen, A. Gödecke, A. Bottermann, K.
Description	Author Affiliation: Emde B ( Heinrich-Heine University Düsseldorf. Barbara.Emde@uni-duesseldorf.de.)
Abstract	The quantification of fibrotic tissue is an important task in the analysis of cardiac remodeling. The use of established fibrosis staining techniques is limited on frozen cardiac tissue sections due to a reduced color contrast compared to paraffin embedded sections. We therefore used FITC-labeled wheat germ agglutinin (WGA), which marks fibrotic tissue in comparable quality as the established picrosirius red (SR) staining, for the staining of post myocardial infarction scar tissue. The fibrosis amount was quantified in a histogram-based approach using the non-commercial image processing program ImageJ. Our results clearly demonstrate that WGA-FITC is a suitable marker for cardiac fibrosis in frozen tissue sections. In combination with the histogram-based analysis, this new quantification approach is i) easy and fast to perform; ii) suitable for raw frozen tissue sections; and iii) allows the use of additional antibodies in co-immunostaining.
File Format	HTM / HTML
ISSN	1121760X
e-ISSN	20388306
DOI	10.4081/ejh.2014.2448
Journal	European Journal of Histochemistry
Issue Number	4
Volume Number	58
Language	English
Publisher	PagePress
Publisher Date	2014-12-17
Publisher Place	Italy
Access Restriction	Open
Subject Keyword	Discipline Histocytochemistry Fluorescein-5-isothiocyanate Analogs & Derivatives Myocardial Infarction Myocardium Staining And Labeling Wheat Germ Agglutinins Chemistry Animals Fibrosis Immunohistochemistry Mice Metabolism Pathology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Biophysics

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Wheat Germ Agglutinin Staining as a Suitable Method for Detection and Quantification of Fibrosis in Cardiac Tissue after Myocardial Infarction

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Wheat germ agglutinin staining as a suitable method for detection and quantification of fibrosis in cardiac tissue after myocardial infarction.

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Wheat Germ Agglutinin Staining as a Suitable Method for Detection and Quantification of Fibrosis in Cardiac Tissue after Myocardial Infarction

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Wheat germ agglutinin staining as a suitable method for detection and quantification of fibrosis in cardiac tissue after myocardial infarction.