NDLI: Evaluation of interferon response induced by anti-myostatin shRNA constructs in goat (Capra hircus) fetal fibroblasts by quantitative real time-polymerase chain reaction.

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Evaluation of interferon response induced by anti-myostatin shRNA constructs in goat (Capra hircus) fetal fibroblasts by quantitative real time-polymerase chain reaction.

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Evaluation of interferon response induced by anti-myostatin shRNA constructs in goat (Capra hircus) fetal fibroblasts by quantitative real time-polymerase chain reaction.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Singh, Nirmal Kumar Singh, Sanjeev Jain, Sudhir Kumar Sarkhel, Bikash Chandra
Description	Country affiliation: India Author Affiliation: Singh NK ( Animal Biotechnology Centre, Madhya Pradesh Pashu Chikitsa Vigyan Vishwavidyalaya Campus, Adhartal, Jabalpur, India.)
Abstract	RNAi is an evolutionary conserved, highly efficient, and cost effective technique of gene silencing. It holds considerable promise and success has been achieved both in vitro and in vivo experiments. However, it is not devoid of undesirable side effects as dsRNA can trigger the immune response and can also cause non-specific off-target gene silencing. In the present study, silencing of myostatin gene, a negative regulator of myogenesis, was evaluated in caprine fetal fibroblasts using three different shRNA constructs. Out of these three constructs, two constructs sh1 and sh2 showed, 72% and 50% reduction (p<0.05) of myostatin mRNA, respectively. Efficient suppression (42-86%) of MSTN gene (p<0.05) was achieved even by reducing the concentration of shRNA constructs. The induction of classical interferon stimulated gene (Oligoandenylate Synthetase-1, OAS-1) was studied to analyze the immune response against shRNAs. Notably, a reduction in the potency of shRNAs to induce interferon response was observed at lower concentration for OAS1 gene. The results obtained in the study would be helpful in the abrogation of the bystander effects of RNAi for long term stable expression of anti-MSTN expression constructs in the muscle.
File Format	HTM / HTML
ISSN	10495398
Issue Number	3
Volume Number	23
e-ISSN	15322378
Journal	Animal Biotechnology
Language	English
Publisher	Taylor & Francis
Publisher Date	2012-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Zoology Discipline Biotechnology Goats Genetics Immunology Interferons Biosynthesis Myostatin Antagonists & Inhibitors 2',5'-oligoadenylate Synthetase Animals Base Sequence Biotechnology Cells, Cultured Dna Primers Fetus Fibroblasts Gene Knockdown Techniques Growth & Development Muscle Development Rna Interference Rna, Small Interfering Real-time Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Bioengineering Biotechnology

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