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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ueno, Yuko Furukawa, Kazuaki Tin, Andrew Hibino, Hiroki |
| Description | Author Affiliation: Ueno Y ( NTT Basic Research Laboratories, NTT Corp.) |
| Abstract | We propose a molecular design for a biomolecular probe to realize an on-chip graphene oxide (GO) aptasensor with enhanced sensitivity. Here, GO works as an excellent acceptor for fluorescence resonance energy transfer. We inserted a rigid double-stranded DNA as a spacer between the GO surface and the aptamer sequence to extend the distance between a fluorescence dye and the GO surface during molecular recognition. We examined the dependence of the sensitivity on the length of the spacer quantitatively by using a 2×2 linear-array aptasensor. We used the modified aptamer with 10 and 30 base pair (bp) double-stranded DNA spacers. The signal with a 30bp-spacer was about twice as strong that with a 10bp-spacer as regards both thrombin and prostate specific antigen detections. The improvement in the sensitivity was supported by a model calculation that estimated the effect of spacer length on fluorescence recovery efficiency. |
| File Format | HTM / HTML |
| ISSN | 09106340 |
| e-ISSN | 13482246 |
| Journal | Analytical Sciences |
| Issue Number | 9 |
| Volume Number | 31 |
| Language | English |
| Publisher | The Japan Society for Analytical Chemistry |
| Publisher Date | 2015-01-01 |
| Publisher Place | Japan |
| Access Restriction | Open |
| Subject Keyword | Discipline Analytical Discipline Chemistry Techniques Aptamers, Nucleotide Genetics Biosensing Techniques Chemistry Fluorescence Resonance Energy Transfer Graphite Microchip Analytical Procedures Oxides Dna Probes Limit Of Detection Prostate-specific Antigen Surface Properties Thrombin |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry |
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