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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Burlibasa, Liliana Zarnescu, Otilia Cucu, Natalia Gavrila, Lucian |
| Description | Country affiliation: Romania Author Affiliation: Burlibasa L ( Institute of Genetics and Developmental Biology Department, Faculty of Biology, University of Bucharest, Bucharest, Romania. liliana_burlibasa@yahoo.com.au) |
| Abstract | Oogenesis is a critical event in the formation of female gametes, whose role in development is to transfer genomic information to the next generation. During this process, the gene expression pattern changes dramatically concomitant with genome remodelling, while genomic information is stably maintained. The aim of the present study was to investigate the chromatin architecture in newt oocytes. Using fluorescence microscopy, as well as transmission electron microscopy (TEM), immunohistochemical method and RE-ChIP assay, some peculiar aspects of chromatin and chromosome organization and evolution in crested newt oogenesis were investigated. We focussed our investigations on detection of certain epigenetic modifications (H4 hyperacetylation, H2A ubiquitinylation and cytosine methylation) at the rRNA gene (18S-5.8S-28S) promoter region. Our findings suggest that there is an involvement of some epigenetic modifications as well as of linker histone variants in chromatin architecture dynamics during crested newt oogenesis. |
| File Format | HTM / HTML |
| ISSN | 09671994 |
| Issue Number | 4 |
| Volume Number | 16 |
| e-ISSN | 14698730 |
| Journal | Zygote |
| Language | English |
| Publisher | Cambridge University Press |
| Publisher Date | 2008-11-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Embryology Chromatin Physiology Epigenesis, Genetic Oogenesis Acridine Orange Animals Cell Nucleolus Ultrastructure Isolation & Purification Coloring Agents Electrophoresis, Polyacrylamide Gel Female Genome Histones Microscopy, Electron Oocytes Cytology Triturus Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology |
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