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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bonnet-Garnier, Amelie Feuerstein, Prisca Chebrout, Martine Fleurot, Renaud Jan, Habib-Ullah Debey, Pascale Beaujean, Nathalie |
| Description | Country affiliation: France Author Affiliation: Bonnet-Garnier A ( UMR1198 Biologie du Développement et Reproduction, Institut National de la Recherche Agronomique, Jouy-en-Josas, France. amelie.bonnet-garnier@jouy.inra.fr) |
| Abstract | During the final step of oogenesis, the oocyte nucleus is subject to large-scale modifications that correlate with transcriptional silencing. While oocytes with dense chromatin around the nucleolus are silent (SN, surrounded nucleolus), oocytes with uncondensed chromatin (NSN, non-surrounded nucleolus) are transcriptionally active. It is believed that epigenetic mechanisms that participate in gene expression regulation could play a role in this event. In this context, we examined the behaviour of heterochromatin and related histone modifications during the NSN to SN transition by immunostaining. Using fluorescent in situ hybridization on three dimensional-preserved nuclei (3D-FISH), we also studied the distribution of centromeric, pericentromeric and ribosomal (rDNA) sequences in relation to the nucleolus (also called the nucleolus-like body, NLB). We observed that in NSN-type oocytes, pericentromeric heterochromatin is aggregated within chromocenters. In SN-type oocytes, pericentromeric heterochromatin and centromeres form a discontinuous ring around the NLB. rDNA sequences, which initially present a pearl necklace structure, gather together in seven highly condensed foci at the NLB periphery. H3K9me3 and H4K20me3 heterochromatin marks clearly label chromocenters, whereas H3K4me3 and H4K5ac are totally excluded from heterochromatin regions, even in the very compact SN-nuclei. Remarkably, H3K27me3 displays an intermediate behavior. It appears that GV oocyte nuclei exhibit a specific epigenetic landscape. Histone modifications, related to both active and repressive chromatin structures, seem to follow the large-scale chromatin movements that occur during the NSN to SN transition. We also demonstrate that, while heterochromatin regions re-localize around the NLB, rDNA sequences adopt a highly compact structure in SN-type oocytes. |
| File Format | HTM / HTML |
| ISSN | 02146282 |
| e-ISSN | 16963547 |
| Journal | The International Journal of Developmental Biology |
| Issue Number | 10-12 |
| Volume Number | 56 |
| Language | English |
| Publisher | University of the Basque Country Press |
| Publisher Date | 2012-01-01 |
| Publisher Place | Spain |
| Access Restriction | Open |
| Subject Keyword | Discipline Embryology Discipline Biology Cell Nucleus Genetics Epigenesis, Genetic Genome Oocytes Metabolism Animals Cell Nucleolus Centromere Chromatin Dna, Ribosomal Epigenomics Heterochromatin Histones In Situ Hybridization, Fluorescence Methylation Mice Mice, Inbred C57bl Mice, Inbred Cba Microscopy, Fluorescence Cytology Oogenesis Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Developmental Biology Embryology |
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