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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kjaerulff, Søren Nielsen, Olaf |
| Description | Country affiliation: Denmark Author Affiliation: Kjaerulff S ( Department of Biology, University of Copenhagen, DK-2220, Copenhagen N, Denmark.); Nielsen O ( Chemometec, DK-3450, Allerod, Denmark.) |
| Abstract | We here describe an IPTG-inducible system that reveals that the lac repressor alone can function as a potent transmodulator to regulate gene expression in the fission yeast, Schizosaccharomyces pombe. This expression system is a derivative of the Sz. pombe nmt promoter, which normally is strongly repressed by thiamine. With appropriate positioning of a lac operator site (lacO) downstream of the TATA-box, we show that gene expression from a chimeric nmt::lacO promoter can be regulated by the lac repressor up to two orders of magnitude in response to IPTG. The chimeric nmt::lacO promoter is rapidly induced and when GFP is used as a reporter; almost full induction is achieved 40 min after the addition of IPTG. Like the wild-type nmt promoter, the chimeric nmt::lacO is repressed by thiamine. This allows expression in a short and defined window, e.g. the S-phase of a synchronized cell population, by first adding IPTG to turn on expression, followed by addition of thiamine to switch off expression. |
| File Format | HTM / HTML |
| ISSN | 0749503X |
| Issue Number | 6 |
| Volume Number | 32 |
| e-ISSN | 10970061 |
| Journal | Yeast |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2015-06-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Gene Expression Regulation, Fungal Isopropyl Thiogalactoside Metabolism Promoter Regions, Genetic Schizosaccharomyces Pombe Proteins Genetics Schizosaccharomyces Repressor Proteins Growth & Development Thiamine Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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