NDLI: Multi-development-HPTLC method for quantitation of hyoscyamine, scopolamine and their biosynthetic precursors in selected solanaceae plants grown in natural conditions and as in vitro cultures.

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Multi-development-HPTLC method for quantitation of hyoscyamine, scopolamine and their biosynthetic precursors in selected solanaceae plants grown in natural conditions and as in vitro cultures.

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Multi-development-HPTLC method for quantitation of hyoscyamine, scopolamine and their biosynthetic precursors in selected solanaceae plants grown in natural conditions and as in vitro cultures.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jaremicz, Zbigniew Luczkiewicz, Maria Kisiel, Mariusz Zárate, Rafael El Jaber-Vazdekis, Nabil Migas, Piotr
Description	Country affiliation: Poland Author Affiliation: Jaremicz Z ( Department of Pharmacognosy, Medical University of Gdansk, al. gen. J. Hallera 107, 80-416, Gdansk, Poland.)
Abstract	INTRODUCTION: Hyoscyamine and scopolamine, anti-cholinergic agents widely used in medicine, are typically obtained from plants grown under natural conditions. Since field cultivation entails certain difficulties (changeable weather, pests, etc.), attempts have been made to develop a plant in vitro culture system as an alternative source for the production of these compounds. During experiments to locate the limiting steps in the biotechnological procedure, it is important to monitor not only the levels of the final products but also the changes in the concentration of their precursors. OBJECTIVE: To develop a HPTLC method for the separation and quantitation of the main tropane alkaloids hyoscyamine and scopolamine, their respective direct precursors littorine and anisodamine, and cuscohygrine, a product of a parallel biosynthetic pathway that shares a common precursor (N-methyl-∆(1) -pyrrolium cation) with tropane alkaloids. METHODS: Using alkaloid extracts from Atropa baetica hairy roots, different TLC chromatographic systems and developing procedures were investigated. RESULTS: Full separation of all compounds was obtained on HPTLC Si60 F254 plates preconditioned with mobile phase vapours (chloroform:methanol:acetone:25% ammonia ratios of 75:15:10:1.8, v/v/v/v). The chromatograms were developed twice (at distances of 4.0 and 3.0 cm) in a Camag twin trough chamber and visualised with Dragendorff's reagent. Densitometric detection (λ = 190 and 520 nm) was used for quantitative analyses of the different plant samples. CONCLUSION: This method can be recommended for quantitation of hyoscyamine, scopolamine, anisodamine, littorine and cuscohygrine in different plant material (field grown vs. in vitro cultures).
File Format	HTM / HTML
ISSN	09580344
Issue Number	1
Volume Number	25
e-ISSN	10991565
Journal	Phytochemical Analysis
Language	English
Publisher	Wiley
Publisher Date	2014-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Botany Discipline Chemistry Atropine Derivatives Analysis Chromatography, Thin Layer Methods Hyoscyamine Scopolamine Hydrobromide Solanaceae Chemistry Solanaceous Alkaloids Acetone Analogs & Derivatives Atropa Metabolism Plant Roots Cytology Pyrrolidines Reproducibility Of Results Tissue Culture Techniques Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Plant Science Drug Discovery Analytical Chemistry Biochemistry Molecular Medicine Food Science Complementary and Alternative Medicine

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