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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hayashi, Tetsutaro Shibata, Norito Okumura, Ryo Kudome, Tomomi Nishimura, Osamu Tarui, Hiroshi Agata, Kiyokazu |
| Description | Country affiliation: Japan Author Affiliation: Hayashi T ( RIKEN Center for Developmental Biology, Chuo-ku, Kobe 650-0047, Japan.) |
| Abstract | To achieve an integrated understanding of the stem cell system of planarians at both the cellular and molecular levels, we developed a new method by combining 'fluorescent activated cell sorting (FACS) index sorting' analysis and single-cell reverse transcription-polymerase chain reaction (RT-PCR) to detect the gene expression and cell cycle state of stem cells simultaneously. Single cells were collected using FACS, and cDNAs of each cell were used for semi-quantitative RT-PCR. The results were plotted on the FACS sorting profile using the 'index sorting' function, which enabled us to analyze the gene expression in combination with cell biological data (such as cell cycle phase) for each cell. Here we investigated the adult stem cells of planarians using this method and obtained findings suggesting that the stem cells might undergo commitment during S to G2/M phase. This method could be a powerful and straightforward tool for examining the stem cell biology of not only planarians but also other organisms, including vertebrates. |
| File Format | HTM / HTML |
| ISSN | 00121592 |
| Issue Number | 1 |
| Volume Number | 52 |
| e-ISSN | 1440169X |
| Journal | Development, Growth & Differentiation |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2010-01-01 |
| Publisher Place | Japan |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Embryology Discipline Biology Gene Expression Profiling Planarians Cytology Stem Cells Metabolism Animals Cell Division Genetics Cell Separation Methods Cluster Analysis Female Flow Cytometry G2 Phase Genes, Helminth Helminth Proteins In Situ Hybridization Male Radiation Effects Proliferating Cell Nuclear Antigen Research Design Reverse Transcriptase Polymerase Chain Reaction S Phase Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Medicine |
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